高迁移率族蛋白B1对小鼠调节性T细胞免疫功能的影响及机制研究的开题报告Title：EffectandMechanismofHighMobilityGroupBox1ProteinonRegulatoryTCellImmuneFunctioninMiceIntroduction：RegulatoryTcells(Tregs)playimportantrolesinmaintainingimmunetoleranceandpreventingautoimmunediseases.DysregulationofTregfunctioncanleadtothedevelopmentofinflammatoryandautoimmunedisorders.Highmobilitygroupbox1protein(HMGB1)isaDNAbindingproteinthathasbeenshowntohavebothpro-inflammatoryandanti-inflammatoryeffects.However,theroleofHMGB1inTregfunctionisnotwellunderstood.Inthisstudy,weaimtoinvestigatetheeffectandmechanismofHMGB1onTregimmunefunctioninmice.Methods：1.IsolationandcultureofTregsfrommousespleenandlymphnodes.2.InvitroassessmentofTregfunctionbyflowcytometry,ELISA,andqPCR.3.AnimalexperimentstoevaluatetheeffectofHMGB1onTregfunctioninvivo.4.MechanisticstudiesusingsiRNA-mediatedknockdownofHMGB1receptorsanddownstreamsignalingpathways.ExpectedResults：WeexpecttodemonstratethatHMGB1playsanimportantroleinregulatingTregfunctionandimmunehomeostasisinmice.OurresultsmayofferinsightsintothepotentialdevelopmentofHMGB1-basedtherapiesfortreatingautoimmunediseases.Conclusion：ThisstudyaimstoinvestigatetheeffectandmechanismofHMGB1onTregfunctioninmice.Theresultsmayprovidenewinsightsintotheregulationofimmunehomeostasisandthedevelopmentofnewtherapeuticapproachesforautoimmunediseases.