NSCs三维培养及其微流控动态模型的构建的开题报告【摘要】神经干细胞（NSCs）的三维培养和微流控技术对于理解神经系统的发育、重建和疾病研究具有重要意义。本文旨在探索NSCs三维培养及其微流控动态模型的构建，以实现研究神经系统发育和疾病模拟。文中首先介绍NSCs的性质及其三维培养技术，描述神经元、星形胶质细胞等细胞类型形成的神经元球的构建过程。接着，详细介绍微流控技术的原理和工作方式，并阐述微流控模型可用于调控NSCs三维结构的形态和功能。最后，提出本文的研究方案，通过建立NSCs三维培养和微流控动态模型，在调控神经元球内不同细胞类型的比例和增殖速率的基础上，研究神经系统发育和疾病模拟，实现对神经系统发育和疾病的深入理解和探究。【关键词】NSCs；三维培养；微流控；神经元球；神经系统发育；疾病模拟【Abstract】Three-dimensionalcultureandmicrofluidictechnologyofneuralstemcells(NSCs)areofgreatsignificanceforunderstandingthedevelopment,reconstructionanddiseaseresearchofthenervoussystem.ThispaperaimstoexploretheconstructionofNSCsthree-dimensionalcultureandmicrofluidicdynamicmodeltostudythedevelopmentanddiseasesimulationofthenervoussystem.Initially,thepropertiesofNSCsandtheirthree-dimensionalculturetechniqueswereintroduced,andtheconstructionprocessofneuronalspheresformedbyvariouscelltypessuchasneuronsandastrocyteswasdescribed.Then,theprincipleandworkingmodeofmicrofluidictechnologywereelaborated,anditwasexplainedthatmicrofluidicmodelscouldbeusedtoregulatethemorphologyandfunctionofNSCsthree-dimensionalstructures.Finally,theresearchplanofthispaperwasproposed.ByestablishingNSCsthree-dimensionalcultureandmicrofluidicdynamicmodels,theproportionandproliferationrateofdifferentcelltypesintheneuronalspherewerestudiedtounderstandandexplorethedevelopmentanddiseaseofthenervoussystemcomprehensively.【Keywords】NSCs;three-dimensionalculture;microfluidic;neuronalsphere;nervoussystemdevelopment;diseasesimulation