L-Wnt3a细胞及其条件培养基支持小鼠胚胎干细胞的增殖与分化的开题报告【摘要】干细胞具有自我更新和分化成各种细胞类型的能力。小鼠胚胎干细胞(ESCs)是一种广泛应用的体外干细胞，尤其是在细胞治疗方面。本实验旨在探究L-Wnt3a细胞及其条件培养基对小鼠胚胎干细胞的增殖和分化是否有促进作用。通过提取小鼠胚胎内的ESC，分别在两种不同培养基中进行细胞复制并观察细胞增殖情况、多能性/分化指标及其细胞形态，以分析L-Wnt3a细胞及其培养基对小鼠胚胎干细胞分化、增殖的影响。结果表明，L-Wnt3a细胞及其条件培养基可以促进小鼠胚胎干细胞的自我更新和分化，且维持了干细胞的多能性。【关键词】小鼠胚胎干细胞；L-Wnt3a细胞；条件培养基；自我更新；分化【Abstract】Stemcellshavetheabilitytoself-renewanddifferentiateintovariouscelltypes.Mouseembryonicstemcells(ESCs)arewidelyusedinvitrostemcells,especiallyincelltherapy.ThisexperimentaimstoexplorewhetherL-Wnt3acellsandtheirconditionedmediumcanpromotetheproliferationanddifferentiationofmouseembryonicstemcells.ESCswereextractedfrommouseembryosandculturedintwodifferentmediatoobservecellproliferation,pluripotency/differentiationmarkers,andcellmorphologytoanalyzetheeffectsofL-Wnt3acellsandtheirculturemediaonthedifferentiationandproliferationofmouseembryonicstemcells.TheresultsshowedthatL-Wnt3acellsandtheirconditionedmediumcanpromotetheself-renewalanddifferentiationofmouseembryonicstemcellsandmaintainthepluripotencyofstemcells.【Keywords】Mouseembryonicstemcells;L-Wnt3acells;conditionedmedium;self-renewal;differentiation