编号：时间：2021年x月x日书山有路勤为径，学海无涯苦作舟页码：第页共NUMPAGES39页第PAGE\*MERGEFORMAT39页共NUMPAGES\*MERGEFORMAT39页ExperimentalStudyonInfluenceofBaoShengDecoctiononTestisTissueandSpermofSpermatogenicLesionLetdowninMiceMajor:ClinicalBasisofTCMPostgraduate:ZhouXiaoPingTutor:Prof.DaiJingChengABSTRACTObjective:ToinvestigatetheinfluenceofBaoShengDecoction(givenBCD)onspermlesionletdowninmiceandprovideddataforfurtherstudyandclinicalpractice.Methods:Thetestisesofmalemiceweretakenintothewarmbathin43℃within20minterschangingthespermatogeniclesionletdownmodel,andthosemiceswererandomlydividedintothreegroups,respectiveigBSD,WuZiYanZongDecoction(givenWZYZD)andNormalSalineandhavingtakenmedicinefortwoweeks.Anothermiceswasbuiltedthenormalgroup.Thetesticulartissuewasobservedaswellandmeasurethenumber,motilityofsperms,theweightofthetesticles,epididymisandbody.Result:1.ThepathologyoftheinjuredtesticularorganswererestoredremarkablybyBSDgroupandbetterthanthatinWZYZDgroupandModelgroupinmice.2.ThenumberandmotilityofspermscouldremarkablyraisebyBSDgroupthanthatinWZYZDgroup(p<0.01)inmice.3.BSDgroupcouldraisetheweightofthetestisandEpididymisbetterthanthatinWZYZDgroup(0.01<p<0.05)andModelgroup(p<0.01).Conclusions:BSDcouldremarkablypromotethespermatogeneticfunctionoftestisandcouldremarkablyrestorethepathologyoftheinjuredtesticularorgansinmice.ThesefunctionswereledbyBSDgroupbetterthanthatbyWZYZWgroupandModelgroup.MESH:BaoShengDecoction/pharmacologySpermatogeniclessionletdown,experimental/tcdtherTesticularorgans/pathTestisnumber/drugeffMotility/drugeffTestisweight/drugeffBodyweighy/drugeffEpididymis/drugeffMice宝生汤对生精障碍小鼠睾丸组织及精子影响的实验研究专业：中医临床基础研究生：周小平导师：戴锦成教授摘要目的：研究宝生汤对生精障碍模型小鼠的影响，为临床应用和进一步研究宝生汤提供客观的实验和理论依据。方法：雄性小鼠，把其睾丸浸没于43℃温水浴20分钟造模，随机分成三组，分别灌服宝生汤（简称宝生组），五子衍宗汤（简称五子组）和生理盐水（简称模型组），连续2周。另设正常组，常规喂养相同时间。处死后，睾丸组织做电镜及光镜病理切片；检测精子密度、活动率；称睾丸、附睾的重量以及动物体重。结果：1、宝生汤能明显促进损伤睾丸病理恢复正常，优于五子衍宗组及模型组；2、宝生汤能明显提高小鼠精子活动率，优于五子组（p<0.01）及正常组(0.01<p<0.05)；增加小鼠精子密度优于五子组(p<0.01)；3、宝生汤能增加小鼠睾丸和附睾重量，优于五子组(0