骨髓间质干细胞体内突变致瘤及其肿瘤干细胞分离与生物学特性研究的开题报告摘要：骨髓间质干细胞(BM-MSCs)是一种多能的成体干细胞，能够分化成骨细胞、软骨细胞和脂肪细胞。然而，越来越多的研究表明，BM-MSCs也可以通过体内突变致瘤。本文将针对BM-MSCs的体内突变致瘤现象以及相关的肿瘤干细胞分离和生物学特性进行研究。我们将使用流式细胞仪和肿瘤干细胞特异性标记物(CD24和CD44)来分离BM-MSCs中的肿瘤干细胞。同时，我们将采用体外培养和肿瘤形成试验来评估这些肿瘤干细胞的生物学特性。这项研究有助于更好地理解BM-MSCs的潜在致瘤风险，并对肿瘤治疗中的干细胞治疗提供新的思路和方法。关键词：骨髓间质干细胞；体内突变致瘤；肿瘤干细胞；生物学特性；干细胞治疗Abstract：Bonemarrowmesenchymalstemcells(BM-MSCs)areamultipotentadultstemcellsthatcandifferentiateintoosteoblasts,chondrocytes,andadipocytes.However,increasingevidencesuggeststhatBM-MSCscanalsoundergosomaticmutationsandgiverisetotumorsinvivo.Thisreportaimstostudythephenomenonofinvivomutation-inducedtumorigenesisinBM-MSCsandtherelatedisolationandbiologicalcharacteristicsoftumorstemcells.Wewilluseflowcytometryandtumorstemcell-specificmarkers(CD24andCD44)toisolatethetumorstemcellsinBM-MSCs.Meanwhile,wewillevaluatethebiologicalcharacteristicsofthesetumorstemcellsusinginvitrocultureandtumorformationassays.ThisresearchwillhelptobetterunderstandthepotentialtumorigenicrisksofBM-MSCsandprovidenewideasandmethodsforstemcelltherapyincancertreatment.Keywords:bonemarrowmesenchymalstemcells;invivomutation-inducedtumorigenesis;tumorstemcells;biologicalcharacteristics;stemcelltherapy.