17种氨基酸对水分胁迫下大豆幼苗抗旱性的影响的中期报告摘要本研究旨在探究不同氨基酸对水分胁迫下大豆幼苗抗旱性的影响。将大豆种子在含有不同氨基酸的培养液中萌发，待幼苗生长至二叶期后，将其移植到含有不同浓度PEG-6000的培养液中进行水分胁迫处理。测定干旱处理前后幼苗体重、相对水分含量、叶绿素含量以及超氧化物歧化酶(SOD)、过氧化物酶(POD)等抗氧化酶活性。结果显示，17种氨基酸均能够提高大豆幼苗的生长速度、相对水分含量和叶绿素含量，且抗氧化酶活性也有所增加。其中，天冬氨酸、丝氨酸、甘氨酸和苏氨酸对大豆幼苗的抗旱性具有显著的促进作用；而半胱氨酸、色氨酸和赖氨酸则对大豆幼苗的抗旱性产生了负面影响。综合来看，本研究结果表明，不同氨基酸对大豆幼苗的抗旱性产生了不同程度的影响，未来可以通过调控氨基酸代谢途径来提高大豆的抗旱性。关键词：氨基酸；水分胁迫；大豆幼苗；抗旱性；抗氧化酶活性AbstractTheaimofthisstudywastoinvestigatetheeffectsofdifferentaminoacidsonthedroughtresistanceofsoybeanseedlingsunderwaterstress.Soybeanseedsweregerminatedinculturesolutionscontainingdifferentaminoacids,andaftertheseedlingsgrewtothesecondleafstage,theyweretransplantedintoculturesolutionscontainingdifferentconcentrationsofPEG-6000forwaterstresstreatment.Thedryweight,relativewatercontent,chlorophyllcontentandantioxidantenzymeactivitiessuchassuperoxidedismutase(SOD)andperoxidase(POD)weremeasuredbeforeandafterdroughttreatment.Theresultsshowedthatall17aminoacidscouldincreasethegrowthrate,relativewatercontentandchlorophyllcontentofsoybeanseedlings,andtheactivityofantioxidantenzymesalsoincreased.Amongthem,asparticacid,serine,glycineandthreoninehadsignificantpromotingeffectsonthedroughtresistanceofsoybeanseedlings;whilecysteine,tryptophanandlysinehadnegativeeffectsonthedroughtresistanceofsoybeanseedlings.Overall,theresultsofthisstudyindicatethatdifferentaminoacidshavedifferenteffectsonthedroughtresistanceofsoybeanseedlings,andthedroughtresistanceofsoybeancanbeimprovedbyregulatingaminoacidmetabolismpathwaysinthefuture.Keywords:aminoacids;waterstress;soybeanseedlings;droughtresistance;antioxidantenzymeactivity