钼提高冬小麦抗寒力的生理基础及分子机制的开题报告【摘要】钼是一种必需微量元素，在植物生长中起着重要的作用。本研究以冬小麦为材料，通过大田试验和室内水培试验探究了钼对冬小麦抗寒性的影响。结果表明，钼处理显著提高了冬小麦的抗寒性，且该效应与处理浓度和处理时间呈正相关关系。进一步研究发现，钼处理能够提高冬小麦细胞膜的稳定性，降低脂质过氧化程度，增强细胞对低温逆境的耐受性。同时，RNA测序分析揭示出一些与钼响应相关的基因，包括CYP71C6、CYP79D6、AGL42等，这些基因参与了蛋白质合成、细胞信号传导和生长发育等生命过程。因此，本研究为深入了解冬小麦的抗寒机理提供了一定的基础数据，并为钼作为一种有效的抗寒增产剂的应用提供了理论支持。【关键词】钼；冬小麦；抗寒性；细胞膜稳定性；RNA测序【abstract】Molybdenumisanessentialtraceelementthatplaysanimportantroleinplantgrowth.Usingwinterwheatastheexperimentalmaterial,thisstudyinvestigatedtheeffectofmolybdenumoncoldresistanceofwinterwheatthroughfieldexperimentsandindoorhydroponicexperiments.Theresultsshowedthatmolybdenumtreatmentsignificantlyimprovedthecoldresistanceofwinterwheat,andtheeffectwaspositivelycorrelatedwiththetreatmentconcentrationandtime.Furtherresearchfoundthatmolybdenumtreatmentcouldimprovethecellmembranestabilityofwinterwheat,reducethedegreeoflipidperoxidation,andenhancethetoleranceofcellstolow-temperaturestress.Atthesametime,RNAsequencinganalysisrevealedsomegenesrelatedtomolybdenumresponse,includingCYP71C6,CYP79D6,AGL42,etc.,whichareinvolvedinlifeprocessessuchasproteinsynthesis,cellsignaltransduction,andgrowthanddevelopment.Therefore,thisstudyprovidesabasisforin-depthunderstandingofthecoldresistancemechanismofwinterwheatandprovidestheoreticalsupportfortheapplicationofmolybdenumasaneffectiveanti-coldyield-increasingagent.【keywords】Molybdenum;Winterwheat;Coldresistance;Cellmembranestability;RNAsequencing