VEGF-C基因修饰的淋巴结移植促进淋巴管再生的初步研究的开题报告-第一文库 | 海量文档资源下载与分享平台

在线预览结束，喜欢就下载吧，查找使用更方便

10 金币

下载此文档

/ 2

下载提示文本预览

如果您无法下载资料，请参考说明：

1、部分资料下载需要金币，请确保您的账户上有足够的金币

2、已购买过的文档，再次下载不重复扣费

3、资料包下载后请先用软件解压，在使用对应软件打开

VEGF-C基因修饰的淋巴结移植促进淋巴管再生的初步研究的开题报告Title:APreliminaryStudyonPromotingLymphaticRegenerationbyVEGF-CGeneModifiedLymphNodeTransplantationIntroduction:Lymphaticsystemplaysanimportantroleinmaintainingfluidbalanceandimmunefunctioninthebody.Lymphedema,aconditioncharacterizedbytheaccumulationoflymphaticfluid,isacommoncomplicationincancerpatientswhoundergolymphnodedissection.Currently,thetraditionaltherapyforlymphedemaincludescompressiontherapy,physicaltherapy,andsurgicalintervention.However,thesetherapiesfailtoachievedesirabletherapeuticeffectsduetothelimitedcapacityofthelymphaticsystemtoregenerate.Therefore,noveltherapiesthatcanpromotelymphaticregenerationareurgentlyneeded.VascularendothelialgrowthfactorC(VEGF-C)isacrucialproteinthatplaysasignificantroleinthedevelopmentandmaintenanceofthelymphaticsystem.VEGF-Chasbeenshowntopromotelymphangiogenesis,enhancelymphaticdrainage,andreducetissueedema.Therefore,itisapotentialtherapeutictargetforlymphedematreatment.Hypothesis:WehypothesizethatVEGF-Cgenemodificationcanpromotelymphaticregenerationandimprovelymphedemasymptomsinmicebyenhancinglymphaticvesselgrowthandfunction.WewillinvestigatethishypothesisbyconductingapreliminarystudyofVEGF-Cgene-modifiedlymphnodetransplantation.Methodology:WewilluseamousemodeloflymphedemainducedbylymphnodedissectiontoinvestigatetheeffectofVEGF-Cgene-modifiedlymphnodetransplantationonlymphaticregeneration.Wild-typemiceandVEGF-Cgene-modifiedmicewillbeusedforlymphnodetransplantation.Themicewillbedividedintothreegroups:acontrolgroupreceivingregularlymphnodetransplantation,agroupreceivinglymphnodetransplantationwithVEGF-Cgenemodification,andagroupreceivinglymphnodetransplantationwithsalineinjectionasanegativecontrol.Lymphaticimaging,histologicalanalysis,andWesternblottingwillbeusedtoevaluatetheeffectofVEGF-Coverexpressiononlymphaticvesselgrowth,tissueedema,andlymphedemasymptoms.ExpectedResults:WeexpectthatVEGF-Cgene-modifiedlymphnodetransplantationcanpromotelymphangiogenesis,improvelymphaticdrainag