VerticalChamber诱导的成年雌性食蟹猴抑郁模型的开题报告IntroductionMajordepressivedisorder(MDD),alsoknownasclinicaldepression,isacommonmentalhealthdisorderthataffectsmillionsofpeopleworldwide.MDDischaracterizedbypersistentfeelingsofsadness,hopelessness,andworthlessness,aswellasalossofinterestinactivitiesthatwereonceenjoyable.TheetiologyofMDDiscomplexandmultifactorial,andthedevelopmentofanimalmodelsiscrucialforunderstandingtheunderlyingmechanismsanddevelopingnewtherapeuticstrategies.Thepurposeofthisstudyistoestablishananimalmodelofdepressioninducedbytheverticalchambermethodinadultfemalecrab-eatingmacaques.Themodelwillenableustoinvestigatetheneurobiologicalandbehavioralmechanismsofdepressionandtesttheefficacyofpotentialantidepressanttreatments.MethodsSubjects:Adultfemalecrab-eatingmacaques(Macacafascicularis)wereselectedasthesubjectsforthisstudy.Atotalof16monkeyswererandomlydividedintotwogroups:theexperimentalgroup(n=8)andthecontrolgroup(n=8).Verticalchambermethod:Theverticalchambermethodisawell-establishedbehavioralparadigmusedtoinduceadepressive-likestateinanimals.Inthismethod,theanimalsareplacedinatransparentcylinder,whichisthenrotatedseveraltimes.Theanimalsarethenallowedtorestforaperiodbeforetheprocessisrepeated.Thisprocedureisrepeatedforapredeterminednumberofcycles,usuallyoverseveraldays.Behavioraltests:Thebehavioraltestsusedinthisstudyincludetheforcedswimtest(FST),theopen-fieldtest(OFT),andthetailsuspensiontest(TST).TheFSTandTSTarecommonlyusedteststoassessdepressive-likebehaviorinanimals,whiletheOFTisusedtomeasureanxiety-likebehavior.Neurochemicalanalysis:Afterbehavioraltesting,theanimalswillbesacrificed,andtheirbrainswillbecollectedforneurochemicalanalysis.Thelevelsofdopamine,serotonin,andnorepinephrineinvariousbrainregionswillbemeasuredusinghigh-performanceliquidchromatography.ExpectedoutcomesWehypothesizethattheverticalchambermethodwillinduceadepressive-likestateintheexperimentalgroupcomparedtothecontrolgroup.Behavioraltestswillrevealasignificantdecreaseintheswimmingand