MKK4基因在喉肿瘤中的表达及其临床意义的开题报告摘要：MKK4基因是丝裂原活化蛋白激酶(MAPK)信号通路的关键组分，在癌症发生与发展过程中发挥着重要的作用。本研究旨在探讨MKK4基因在喉肿瘤中的表达及其临床意义。采用实时荧光定量PCR和免疫组织化学方法检测MKK4基因在喉癌组织和对照组织中的表达情况，并分析其与临床病理因素之间的关系。结果显示，在喉癌组织中MKK4基因表达水平显著低于对照组织，且与临床病理因素如淋巴结转移、TNM分期等呈负相关。综合上述结果，可以得出MKK4基因在喉肿瘤发生和发展中起到重要作用的结论，有望成为该疾病的潜在治疗靶点。关键词：MKK4基因；喉肿瘤；表达检测；临床意义Abstract:MKK4geneisakeycomponentofthemitogen-activatedproteinkinase(MAPK)signalingpathway,anditplaysanimportantroleintheoccurrenceanddevelopmentofcancer.TheaimofthisstudywastoinvestigatetheexpressionofMKK4geneinlaryngealcarcinomaanditsclinicalsignificance.Real-timefluorescentquantitativePCRandimmunohistochemistrywereusedtodetecttheexpressionofMKK4geneinlaryngealcarcinomatissuesandcontroltissues,andtoanalyzetherelationshipbetweenitsexpressionandclinicalpathologicalfactors.TheresultsshowedthattheexpressionlevelofMKK4geneinlaryngealcarcinomatissueswassignificantlylowerthanthatincontroltissues,andwasnegativelycorrelatedwithclinicalpathologicalfactorssuchaslymphnodemetastasisandTNMstage.Inconclusion,MKK4geneplaysanimportantroleintheoccurrenceanddevelopmentoflaryngealcarcinoma,andmaybecomeapotentialtherapeutictargetforthisdisease.Keywords:MKK4gene;laryngealcarcinoma;expressiondetection;clinicalsignificance.