5-HMF等中药有效成分抗低氧保护作用及其机理研究的中期报告【摘要】低氧环境对生物体健康有着重要影响。中药中的一些有效成分已被证明具有抗低氧保护作用，但其机理仍不明确。本研究旨在探讨5-HMF等中药有效成分在抗低氧方面的作用机制。实验采用体外低氧培养和酶联免疫吸附法等方法，分析了不同浓度的5-HMF等中药有效成分对低氧条件下对模型细胞SOX2和β-III-Tubulin蛋白表达、ROS水平和ATP含量的影响。结果表明，5-HMF等中药有效成分可以显著降低低氧条件下SOX2和β-III-Tubulin蛋白表达的下降及ROS水平的升高，并提高细胞ATP含量。此外，5-HMF等中药有效成分的抗低氧保护功能与Nrf2信号通路有关，可以促进Nrf2核转位并激活其下游抗氧化基因的表达。这些结果表明，5-HMF等中药有效成分通过激活Nrf2信号通路，降低ROS水平，增加ATP含量等方式，发挥抗低氧保护作用。【关键词】5-HMF；中药有效成分；抗低氧保护；Nrf2信号通路；SOX2；β-III-Tubulin；ROS；ATP【Abstract】Lowoxygenenvironmenthasimportanteffectsonthehealthoforganisms.SomeeffectivecomponentsintraditionalChinesemedicine(TCM)havebeenshowntohaveanti-hypoxiaprotectiveeffects,buttheirmechanismsarestillunclear.Thisstudyaimstoexplorethemechanismof5-HMFandothereffectivecomponentsofTCMinanti-hypoxiaprotection.Invitrohypoxiccultureandenzyme-linkedimmunosorbentassaywereusedtoanalyzetheeffectsofdifferentconcentrationsof5-HMFandothereffectivecomponentsofTCMontheexpressionofmodelcellSOX2andβ-III-Tubulinproteins,ROSlevels,andATPcontentunderlowoxygenconditions.Theresultsshowedthat5-HMFandothereffectivecomponentsofTCMcouldsignificantlyreducethedecreaseinSOX2andβ-III-TubulinproteinexpressionandtheincreaseinROSlevelsunderhypoxicconditions,andincreasecellATPcontent.Inaddition,theanti-hypoxiaprotectivefunctionof5-HMFandothereffectivecomponentsofTCMisrelatedtotheNrf2signalingpathway,whichcanpromoteNrf2nucleartranslocationandactivatetheexpressionofdownstreamantioxidantgenes.Theseresultssuggestthat5-HMFandothereffectivecomponentsofTCMplayananti-hypoxiaprotectiverolebyactivatingtheNrf2signalingpathway,reducingROSlevels,increasingATPcontent,etc.【Keywords】5-HMF;effectivecomponentsofTCM;anti-hypoxiaprotection;Nrf2signalingpathway;SOX2;β-III-Tubulin;ROS;ATP