UBA52与Hsf4b的相互作用及调控的开题报告摘要：UBA52是一种结合核糖体蛋白复合物的蛋白质，并参与蛋白降解和特异性翻译后修饰。Hsf4b是一种基因转录因子，参与眼球透明质的生长和维护。本文旨在探究UBA52与Hsf4b之间的相互作用及其调控机制。首先，通过蛋白质相互作用分析验证了UBA52与Hsf4b之间的物理相互作用；其次，运用免疫印迹法、蛋白质稳定性分析和荧光共聚焦显微镜技术，发现Hsf4b通过促进UBA52的泛素化和降解来调控UBA52的表达水平和功能；最后，通过小鼠模型验证了UBA52与Hsf4b相互作用对眼球透明度和复视能力的影响。关键词：UBA52、Hsf4b、相互作用、调控、眼球透明质Abstract：UBA52isaproteinthatbindstotheribosomalproteincomplexandisinvolvedinproteindegradationandspecifictranslatorpost-modification.Hsf4bisagenetranscriptionfactorinvolvedinthegrowthandmaintenanceofeyetransparency.ThisarticleaimstoexploretheinteractionbetweenUBA52andHsf4banditsregulatorymechanism.Firstly,thephysicalinteractionbetweenUBA52andHsf4bwasverifiedbyproteininteractionanalysis.Secondly,throughimmunoblotting,proteinstabilityanalysisandfluorescenceco-localizationmicroscopytechnology,itwasfoundthatHsf4bregulatestheexpressionandfunctionofUBA52bypromotingUBA52ubiquitinatinganddegradation.Finally,amousemodelwasusedtoverifytheeffectoftheinteractionbetweenUBA52andHsf4boneyetransparencyandvisualfatigue.Keywords:UBA52,Hsf4b,interaction,regulation,eyetransparency.