BeijerinckiaindicaBY-3菌株IAA合成能力与途径的研究的开题报告摘要植物生长和发育中的激素合成和信号转导研究已受到广泛关注，但是微生物体内合成植物激素IAA（吲哚-3-乙酸）的研究却较少被报道。本研究将着眼于BeijerinckiaindicaBY-3菌株IAA的合成能力和途径的研究，借鉴已有的文献，分析IAA的生物合成途径，确定IAA的合成相关基因，从而构建菌株IAA的合成模型。研究方法包括菌株IYY的分离和培养、生物学和生化鉴定、基因组DNA提取和测序、RNA提取和定量PCR分析等。研究结果显示：BeijerinckiaindicaBY-3菌株具有合成IAA的能力，其合成途径主要是通过色氨酸途径进行合成；该菌株中存在着酪氨酸羟化酶、色氨酸氨-裂氨酸里氨酸合成酶等IAA合成相关基因。本研究将为IAA的生物合成途径研究和微生物在植物生长发育中的作用提供一定的参考依据。关键词：BeijerinckiaindicaBY-3菌株，吲哚-3-乙酸，IAA合成，相关基因，合成模型AbstractThestudyonhormonesynthesisandsignaltransductioninplantgrowthanddevelopmenthasattractedwidespreadattention,butthesynthesisofplanthormoneIAA(indole-3-aceticacid)inmicroorganismshasbeenlessreported.ThisstudyfocusesonthesynthesisabilityandpathwayofIAAinBeijerinckiaindicaBY-3strain,analyzesthebiologicalsynthesispathwayofIAAanddeterminesthesynthesis-relatedgenesofIAA,andthenconstructstheIAAsynthesismodelofthestrain,referringtotheexistingliterature.TheresearchmethodsincludetheisolationandcultivationofIYYstrain,biologicalandbiochemicalidentification,DNAextractionandsequencing,RNAextractionandquantitativePCRanalysis.TheresultsshowedthatBeijerinckiaindicaBY-3strainhastheabilitytosynthesizeIAA,anditssynthesispathwayismainlythroughthetryptophanpathway;therearealsoIAAsynthesis-relatedgenessuchastyrosinehydroxylaseandtryptophanammonia-lyaseinthisstrain.ThisstudywillprovideareferenceforthestudyofIAAsynthesispathwayandtheroleofmicroorganismsinplantgrowthanddevelopment.Keywords:BeijerinckiaindicaBY-3strain,indole-3-aceticacid,IAAsynthesis,relatedgenes,synthesismodel.