力竭运动过程中大鼠纹状体对皮层运动区电活动的调控作用研究的中期报告IntroductionExercise-inducedfatigue,definedasthedecreaseinphysicalperformanceduringprolongedorintenseexercise,resultsfromacombinationoffactorssuchasdepletionofenergysubstrates,accumulationofmetabolites,andimpairedneuraldrive.Studieshavesuggestedthatthemotorcortexplaysanimportantroleinfatiguedevelopmentandmodulationduringexercise.However,theprecisemechanismsbywhichthemotorcortexinteractswithotherbrainregionstoregulateexercise-inducedfatigueremainunclear.Thestriatum,akeycomponentofthebasalganglia,receivesinputfrommultiplebrainregionsandisinvolvedintheregulationofmovementandothercognitiveprocesses.Previousstudiesinhumansandrodentshaveshownthatthestriatumcontributestothemodulationoffatiguedevelopmentduringexercise.However,theneuralmechanismsunderlyingthestriatalmodulationoffatigueremainpoorlyunderstood.Thepresentstudyaimstoinvestigatetheroleofthestriatuminmodulatingcorticalactivityduringexercise-inducedfatigueinrats.Methods-Animals:EightmaleSprague-Dawleyratswereusedinthisstudy.-Surgery:Allratswereanesthetizedandimplantedwithelectrodestargetingthemotorcortexandthestriatum.-Exerciseprotocol:Ratsweretrainedtorunonamotorizedtreadmillfor30min/dayfor5days/weekfor2weeks.Onthetestday,ratswererunataspeedof15m/minuntilfatigue,definedasthepointatwhichratscouldnolongermaintainthespeedandfelloffthetreadmill.-EEGrecording:Electroencephalogram(EEG)recordingswereobtainedfromthemotorcortexandthestriatumduringtheexerciseprotocol.-Dataanalysis:EEGsignalswereanalyzedusingtime-frequencydecompositionandcoherenceanalysistoassesschangesincorticalactivityandstriatal-corticalinteractionsduringexercise.ResultsPreliminaryresultsindicatethat:-Corticalactivityincreasedduringtheearlyphaseofexercisebutdecreasedduringfatiguedevelopment.-Striatalactivityincreasedduringtheearlyphaseofexerciseandremainedelevatedduringfatigue.-Coherenceanalysisrevealedthatstriatal-corticalinteractionsincreasedduringexerciseandwerepositivelycorrelatedwithexerciseduration.Con