不同时程吗啡依赖对大鼠大脑皮层神经细胞的影响开题报告摘要随着剖析药物成瘾机制和药物成瘾治疗方法的深入，对于阿片类药物的依赖性研究越来越多。然而，对于不同时程啡依赖对大鼠大脑皮层神经细胞的影响的研究尚不充分。本研究的目的是探索不同时程啡依赖对大鼠大脑皮层神经细胞的影响和机制。采用啡类安给微子诱导大鼠成瘾模型，分别在断药0天、7天、14天和21天时取出大脑皮层组织，通过细胞增殖试验和Westernblot检测来探究啡依赖药物对于大鼠大脑皮层神经细胞的影响以及相关机制。本研究的结果有望为阿片类药物成瘾的治疗提供理论和实验基础。关键词：啡依赖；大鼠大脑皮层神经细胞；细胞增殖；Westernblot检测AbstractAstheanalysisofdrugaddictionmechanismanddrugaddictiontreatmentmethodsdeepens,researchonopioiddependenceisincreasing.However,researchontheeffectsofdifferentdurationsofmorphinedependenceoncorticalneuronsinratsisnotsufficient.Thepurposeofthisstudyistoexploretheeffectsandmechanismsofdifferentdurationsofmorphinedependenceoncorticalneuronsinrats.Byinducingrataddictionmodelwithopioidangetics,thecorticaltissuewastakenoutat0days,7days,14daysand21daysafterdrugwithdrawal,respectively,andcellproliferationassayandWesternblotwereusedtoexploretheeffectsofopioiddependencedrugsoncorticalneuronsinratsandrelatedmechanisms.Theresultsofthisstudyareexpectedtoprovidetheoreticalandexperimentalbasisforthetreatmentofopioidaddiction.Keywords:Opioiddependence;Corticalneuronsinrats;Cellproliferation;Westernblotdetection.