附录2培养基1-LB培养基组成Typtone10g/LYeastExtract5g/LNaCl10g/L用5MNaOH调PH=7.0-7.2，定容，高压灭菌15-20min。固体培养基加入1.5％的琼脂。母液成分称量（mg/L）ⅠNH4NO31650KNO31900MgSO4.7H2O370KH2PO4170ⅡKI0.83H3BO36.20MnSO4.1H2O16.9ZnSO4.7H2O8.60CuSO4.5H2O0.025CoCl2.6H2O0.025Na2MoO4.2H2O0.25ⅢFeSO4.7H2O27.8Na2EDTA.2H2O37.3Ⅳ肌醇Inositol100烟酸Nicotinicacid0.5维生素B6PyrodoxineHCl0.5维生素B1ThiamineHCl0.1甘氨酸（氨基乙酸）Glycine2.0ⅤCaCl2.2H2O4402-MS培养基成分表蔗糖Sucrose30.0g琼脂Agar7.0gpH5.7母液成分称量（mg/L）Ⅰ(NH4)2SO4463KNO32830MgSO4.7H2O185KH2PO4400ⅡKI0.8H3BO31.6MnSO4.4H2O4.4ZnSO4.7H2O1.5ⅢFeSO4.7H2O1.390Na2EDTA.2H2O1.865烟酸Nicotinicacid0.5维生素B6PyrodoxineHCl0.5维生素B1ThiamineHCl1.0甘氨酸（氨基乙酸）Glycine2.0ⅤCaCl2.2H2O166蔗糖Sucrose5%琼脂Agar0.7%pH5.73-N6培养基成分4-AA培养基成分母液成分称量(mg/L)ⅠKCl2950MgSO4.7H2O370KH2PO4340ⅡKI0.83H3BO36.20MnSO4.1H2O16.90ZnSO4.7H2O8.60CuSO4.5H2O0.025CoCl2.6H2O0.025Na2MoO4.2H2O0.25ⅢFeSO4.7H2O27.8Na2EDTA.2H2O37.3Ⅳ肌醇Inositol100烟酸Nicotinicacid0.5维生素B6PyrodoxineHCl0.5维生素B1ThiamineHCl0.1L-谷氨酸876（用1NNaOH溶解）L-天冬氨酸266L-精氨酸174.2L-甘氨酸（氨基乙酸）Glycine7.5ⅤCaCl2.2H2O440SolutionI:50mmol/L葡萄糖25mmol/LTris.Cl(PH=8.0)10mmol/LEDTA(PH=8.0)SolutionII:0.4NNaOH2%SDSSolutionIII:60mlKAc，PH,4.8?11.5ml冰乙酸28.5ml水5-质粒提取液组成SolutionI15磅高压灭菌15分钟；SolutionI和SolutionIII保存于4℃，SolutinII现用现配。