NEURALREGENERATIONRESEARCHVolume7,Issue16,June2012www.nrronline.orgCitethisarticleas:NeuralRegenRes.2012;7(16):1213-1219.Morphologicalpropertiesandproliferationanalysisofolfactoryensheathingcellsseededontothree-dimensionalcollagen-heparansulfatebiologicalscaffolds***☆NaLiu,ZhoupingTang,ZhiyuanYu,MinjieXie,YuZhang,ErfangYang,ShabeiXuDepartmentofNeurology,TongjiHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430030,HubeiProvince,ChinaAbstractThisstudyaimedtoexaminethedifferencesinthemorphologicalpropertiesandproliferationofNaLiu☆,Studyingforolfactoryensheathingcellsinthree-dimensionalcultureoncollagen-heparansulfatebiologicaldoctorate,DepartmentofNeurology,TongjiHospital,scaffoldsandintwo-dimensionalcultureoncommonflatcultureplates.TheproliferationrateofTongjiMedicalCollege,olfactoryensheathingcellsinthree-dimensionalculturewashigherthanthatintwo-dimensionalHuazhongUniversityofculture,asdetectedbyanMTTassay.Inaddition,morethanhalfoftheolfactoryensheathingcellsScienceandTechnology,Wuhan430030,Hubeisubculturedusingthetrypsinizationmethodinthree-dimensionalculturedisplayedaspindlyProvince,ChinaSchwanncell-likemorphologywithextremelylongprocesses,whiletheyshowedaflatastrocyte-likemorphologyintwo-dimensionalculture.Moreover,spindle-shapedolfactoryNaLiuandZhoupingTangcontributedequallytothisensheathingcellstendedtoadoptanelongatedbipolarmorphologyunderbothcultureconditions.work.Experimentalfindingsindicatethatthemorphologicalpropertiesandproliferationofolfactoryensheathingcellsinthree-dimensionalcultureoncollagen-heparansulfatebiologicalscaffoldsareCorrespondingauthor:ShabeiXu,M.D.,Professor,betterthanthoseintwo-dimensionalculture.DepartmentofNeurology,TongjiHospital,TongjiMedicalCollege,HuazhongKeyWordsUniversityofScienceandmorphologicalproperties;cellproliferation;biologicalscaffold;olfactoryensheathingcells;Technology,Wuhan430030,three-dimensionalculture;neuralregenerationHubeiProvince,Chinaddjtzp@163.c