HYPERLINK"http://124.16.31.131:8080/Jweb_zzgc"中国组织工程研究HYPERLINK"http://124.16.31.131:8080/Jweb_zzgc/CN/article/showTenYearVolumnDetail.do?nian=2013"2013,HYPERLINK"http://124.16.31.131:8080/Jweb_zzgc/CN/article/showTenYearVolumnDetail.do?nian=2013"Vol.17HYPERLINK"http://124.16.31.131:8080/Jweb_zzgc/CN/volumn/volumn_1156.shtml"Issue(8):1404-1410DOI:10.3969/j.issn.2095-4344.2013.08.013纳米TiO2:C薄膜涂层的生物相容性评价Biocompatibilityofnano-TiO2:Cthinfilmcoating全文:HYPERLINK"http://www.crter.org:8080/Jweb_zzgc/CN/abstract/abstract542.shtml"PDF(599KB)摘要背景：前期实验采用溶胶-凝胶法在纯钛片表面制备了纳米TiO2:C薄膜涂层。目的：评估纳米TiO2:C薄膜涂层的生物相容性。方法：①溶血实验：取新西兰大白兔静脉血，分别加到TiO2-生理盐水、TiO2:C-生理盐水、蒸馏水和生理盐水中。②细胞毒性实验：将TiO2:C浸提液加入对数生长期的L929细胞培养基中。③短期全身毒性实验：将60只BALB/C小白鼠随机分为3组，分别灌胃给予TiO2浸提液、TiO2:C浸提液及生理盐水。④口腔黏膜刺激实验：将牙胶圆片与TiO2:C纳米薄膜涂片分别缝合固定于仓地鼠颊黏膜上。结果与结论：纳米TiO2:C涂层未引起急性溶血，毒性等级为0-1级，未对细胞产生明显毒性，细胞周期未见明显异常；TiO2:C浸提液对小鼠无短期全身毒性；对仓鼠口腔黏膜无刺激性。表明纳米TiO2:C薄膜涂层具有良好的生物安全性。关键词：HYPERLINK"http://www.crter.org:8080/Jweb_zzgc/CN/abstract/abstract542.shtml"生物材料；HYPERLINK"http://www.crter.org:8080/Jweb_zzgc/CN/abstract/abstract542.shtml"纳米生物材料；HYPERLINK"http://www.crter.org:8080/Jweb_zzgc/CN/abstract/abstract542.shtml"材料生物相容性；HYPERLINK"http://www.crter.org:8080/Jweb_zzgc/CN/abstract/abstract542.shtml"纳米二氧化钛；HYPERLINK"http://www.crter.org:8080/Jweb_zzgc/CN/abstract/abstract542.shtml"C；HYPERLINK"http://www.crter.org:8080/Jweb_zzgc/CN/abstract/abstract542.shtml"细胞毒性；HYPERLINK"http://www.crter.org:8080/Jweb_zzgc/CN/abstract/abstract542.shtml"生物相容性；HYPERLINK"http://www.crter.org:8080/Jweb_zzgc/CN/abstract/abstract542.shtml"涂层；HYPERLINK"http://www.crter.org:8080/Jweb_zzgc/CN/abstract/abstract542.shtml"国家自然科学基金；HYPERLINK"http://www.crter.org:8080/Jweb_zzgc/CN/abstract/abstract542.shtml"生物材料图片文章；Abstract：BACKGROUND:Inpreviousexperiments,TiO2:Cthinfilmcoatinghavebeenpreparedonthesurfaceoftitaniumpaperbysol-gelprocess.OBJECTIVE:ToevaluatethebiocompatibilityofnanometerTiO2:Cthinfilmcoating.METHODS:Hemolysiste