PlantSoil(2010)337:273–283DOI10.1007/s11104-010-0523-yREGULARARTICLEAgronomicbiofortificationofBrassicawithselenium—enrichmentofSeMetanditsidentificationinBrassicaseedsandmealMerviM.Seppänen&JuhaKontturi&IsabelLopezHeras&YolandaMadrid&CarmenCámara&HelinäHartikainenReceived:21June2010/Accepted:1August2010/Publishedonline:14August2010#SpringerScience+BusinessMediaB.V.2010AbstractSelenium(Se)isanessentialmicronutrientdeveloped.Seleniumapplicationdidnotaffecttheandiscirculatedtothefoodchainthroughcrops.yieldoroilcontent.HighaccumulationofSeintheBrassicaspeciesareefficientinSeaccumulationandseedsandmeal(1.92–1.96μgSeg−1)wasdetected.thus,goodspeciesforSebiofortificationpurposes.BiotransformationofinorganicSewasevaluatedbyTheresidualfractionobtainedafteroilprocessingofusingHPLC-ICP-MSpreviousenzymatichydrolysisBrassicaseeds,themeal,isanimportantproteinforspeciesextraction.TheSespeciationstudiessourceinanimaldietsandusedinfeedconcentrates.showedthatupto85%ofthetotalSewasSeMetTheaccumulationofsoilorfoliarappliedSeinthewhereasotherSe-specieswerenotdetected.WeseedsandmealofBrassicanapusandB.rapaaswellconcludethattheagronomicbiofortificationofBras-asitseffectsongrowthandyieldformationwassicaspeciescanimprovethenutritivequalityofthestudiedintwofieldexperiments.Also,aHPLC-ICP-proteinrichmealfractionasitcontainssignificantMSbasedmethodfortheidentificationandquantifi-amountofSeMet.cationofSespeciesinBrassicaseedsandmealwasKeywordsBiofortification.Brassicanapus.Brassicarapa.Fertilization.Oilseedrape.Selenium.ResponsibleEditor:HansLambers.SeMet.Speciation.TurniprapeM.M.Seppänen(*):J.KontturiDepartmentofAgriculturalSciences,UniverstityofHelsinki,IntroductionP.O.Box27,00014Helsinki,Finlande-mail:mervi.seppanen@helsinki.fiSelenium(Se)isrecognizedasessentialmicroelementH.Hartikainenforhumansandanimals,mainlyduetoitsantioxidativeDepartmentofFoodandEnvironmentalSciences,propertiesandroleinhormonebalance(SchwartzandUniversityofHelsinki,P.O.Box27,000