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JournalofChromatographyA,1240(2012)96–103ContentslistsavailableatSciVerseScienceDirectJournalofChromatographyAjournalhomepage:www.elsevier.com/locate/chromaHigh-performanceliquidchromatography–massspectrometryandevaporativelight-scatteringdetectortocomparephenolicprofilesofmuscadinegrapesaa,∗bacaQiYou,FengChen,JuliaL.Sharp,XiWang,YuruYou,ChuanjiZhangaDepartmentofFood,NutritionandPackagingSciences,ClemsonUniversity,Clemson,SC29634,USAbDepartmentofMathematicalSciences,ClemsonUniversity,Clemson,SC29634,USAcDepartmentofBiologicalandChemicalEngineering,ZhejiangUniversityofScienceandTechnology,Hangzhou310023,ChinaarticleinfoabstractArticlehistory:Evaporativelightscatteringdetector(ELSD)isconsideredasauniversaldetectorabletodetectalmostanyReceived27January2012compound.ThisstudysuccessfullyestablishedaHPLC-ELSDmethodforthesimultaneousdeterminationReceivedinrevisedform24March2012ofphenolicacids,flavonoidsandanthocyaninsinmuscadinegrape.Twenty-fourphenolics,including5Accepted26March2012anthocyanins,wereidentifiedbyHPLC–UV-MS,tenofthemwereselectedtocalibratetheELSD.ResultsAvailableonline4April2012fromtheELSDquantificationsuggestedthatgallicacid,proanthocyanidins,andellagicacidwerethemainphenolicsinmuscadines.Inaddition,antioxidanttestssuggestedthattheextractsofmuscadineKeywords:grapespossessedstrongantioxidantactivities.Allextractshadahightotalphenoliccontent(TPC).HighMuscadineHPLC-ELSDtotalanthocyaninscontents(TAC)werefoundinNoblemuscadine,andhighproanthocyanidinscontentLC–MS(TPA)intheseedportion.Anthocyanins©2012ElsevierB.V.Allrightsreserved.Phenolics1.IntroductionHowever,phenolicsandanthocyaninsshouldbeanalyzedbyHPLC–MSindifferentionizationmodesbecauseoftheirdiffer-Muscadine(Vitisrotundifolia)isagrapevinespeciesnativetoentchemicalproperties.VeryfewmethodshavebeenreportedthesoutheasternUnitedStates.The
