植物病原诱导性启动子调控元件研究.ppt
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植物病原诱导性启动子调控元件研究.ppt

植物病原诱导性启动子调控元件研究.ppt

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病原物诱导性基因的转录调控植物在受到病原物侵染后,通过多种信号途径引起不同的防卫反应,如产生抗菌性蛋白等。病原应答性基因表达模式的差异是由其启动子结构决定的。(CurrentOpinioninPlantBiology,RushtonandSmossich,1998)Geneexpressionunderthecontrolofinduciblepromotersispreferredinanystrategytoproducetransgenicplantswithtransgene-mediatedimprovementsinresistancetopathogens.(Planta,Mickaeletal,2003)ThestrategyofutilityofinduciblepromotersStrittmatterstrategyA,Fungus-inducedGUSexpressionintransgenictobacco.RelativeGUSactivityinroots,stemsandleavesoftransgenictobaccocarryingpBIG1157(A)andpBIG537B,chimericplasmidsuponfungalinductionareshown.TenindependentT0transformedplantlineswereusedforeachtest.1Leaves,2stems,3roots.(PlantCellReports,2001)Fireblight,causedbythenecrogenicbacteriumErwiniaamylovora(Burr),isoneofthemostdestructivediseasesinpear(Pyrussp.),appleandornamentalplants.Twogenefamilieshavebeenidentifiedintobacco,whoseexpressionisrapidandintenseinresponsetobacterialpathogens:the‘‘hsr’’(‘‘hypersensitivityrelated’’)and‘‘str’’(‘‘sensitivityrelated)genefamiliesfamily.Thestr246Cgeneisolatedfromtobaccobelongstothestrfamily.Thepromoterofthisgeneisnotonlyregulatedbypathogensandelicitors,butalsobyotherenvironmentalanddevelopmentalstimuli,suchashormones,woundingandheavy-metaltreatments.(Planta,2003)AtCMPG1andAtCMPG2,twomembersofthefamilyofCMPG[Cys,Met,Pro,andGly],withhighsequencesimilaritytotheimmediate-earlypathogen-responsivePcCMPG1genefromPetroselinumcrispumwereselectedforanalysisoftheirexpressionmodesanddefense-relatedpromoterelements.Despitethedifferencesinarchitecture,functionaldissectionofthepromoterregionsofsomeofthesegenesiscommonactingelementsandhasprovideduswiththetoolstoidentifydistinctDNA-bindingproteinsrequiredtomodulatetranscription.Apathogen-inducibleplantpromotercontainsmultiplecis-actingelements,onlysomeofwhichmaycontributetothepathogeninduction.elementTheGCC-likeelementsandtheWboxes,twogroupsofpathogen-induciblecis-actingelements,havebeenextensivelyandintensivelystudied.TheGCCBox(AGCCGCC)isoftenfoundinthepromoterregionsofdefensegen