PhoP-PhoQ双组分信号转导系统调控福氏志贺菌毒力的机理研究的开题报告摘要：福氏志贺菌是引起急性肠道感染的主要病原菌之一，其致病机理需要通过一系列的细胞信号转导来实现。PhoP-PhoQ双组分信号转导系统是福氏志贺菌中一种重要的信号传递途径，已被证实可以调控福氏志贺菌的毒力。因此，本文旨在研究PhoP-PhoQ双组分信号转导系统调控福氏志贺菌毒力的机理。在本文中，我们将首先对PhoP-PhoQ双组分信号转导系统的结构和功能进行介绍和分析。接着，我们将讨论PhoP-PhoQ信号转导通路在福氏志贺菌毒力调控中的作用机制。我们将采用多种实验手段，包括基因重组、蛋白质纯化、免疫印迹、荧光定量PCR等，来研究PhoP-PhoQ信号转导通路在福氏志贺菌内部的调节机制及其与福氏志贺菌毒力的关系。由于福氏志贺菌的毒力调控机制的复杂性和重要性，本研究结果对于了解福氏志贺菌的病理生理及其相关病害的治疗和控制具有重要的理论和实践意义。关键词：福氏志贺菌；PhoP-PhoQ双组分信号转导系统；毒力调控；机理研究Abstract:Shigellaflexneriisoneofthemajorpathogenicbacteriathatcausesacuteintestinalinfections,anditspathogenicmechanismrequiresaseriesofcellularsignaltransductionprocesses.ThePhoP-PhoQtwo-componentsignaltransductionsystemisanimportantsignalingpathwayinShigellaflexneri,whichhasbeenshowntocontrolthevirulenceofShigellaflexneri.Therefore,thispaperaimstostudythemechanismofthePhoP-PhoQtwo-componentsignaltransductionsysteminregulatingthevirulenceofShigellaflexneri.Inthispaper,wewillfirstintroduceandanalyzethestructureandfunctionofthePhoP-PhoQtwo-componentsignaltransductionsystem.Then,wewilldiscusstheroleofthePhoP-PhoQsignaltransductionpathwayinregulatingthevirulenceofShigellaflexneri.Wewilluseavarietyofexperimentalmethods,includinggenerecombination,proteinpurification,immunoblotting,fluorescentquantitativePCR,etc.,tostudytheregulatorymechanismofthePhoP-PhoQsignaltransductionpathwayinsideShigellaflexnerianditsrelationshipwiththevirulenceofShigellaflexneri.DuetothecomplexityandimportanceofthevirulenceregulationmechanismofShigellaflexneri,theresultsofthisresearchhaveimportanttheoreticalandpracticalsignificanceforunderstandingthepathophysiologyofShigellaflexneriandthetreatmentandcontrolofrelateddiseases.Keywords:Shigellaflexneri;PhoP-PhoQtwo-componentsignaltransductionsystem;virulenceregulation;mechanismresearch