思路：这篇文章主要是运用转基因技术,失踪技术,对下丘脑中NG2-glia进行不同时期的观察,并对最终分化而成的的带有神经元marker的细胞进行电生理鉴定,最终说明NG2-glia是成体下丘脑中新生神经元的主要来源.NG2-glia:Co-labellingwithNG2andSox2antibodiesindicatedthat37.0%+2.8ofhypothalamicNG2-gliarobustlyexpressSox2(Fig.2A,B,F).DespitethefactthatNG2-gliaaccountedforfewerthan10%ofthetotalSox2+cellpopulation,almostall(92.2%+2.8)ofthehypothalamicBrdU+/Sox2+cellsalsoexpressedNG2(Fig.2A,B,F),suggestingthatNG2-gliaaretheonlysubstantiallyproliferativesubpopulationofSox2+cellsintheadulthypothalamicparenchyma.threetypesofNG2-gliapairsaccordingtoSox2expression:i)substantialSox2expressioninbothcells;ii)notobarelydetectableSox2expressioninbothcells;oriii)Sox2expressionlimitedtoonecell(Fig.2C–E),relatetodifferencesinneuralfates.BC：NG2是下丘脑主要的增值细胞；D-F：30dayspostTX还有NG2细胞表达；L：7天和60天后，双阳性的绝度数量一样，2-7天少突胶质细胞迅速增多——为重组得的保真性提供基础；GH：表面不表达星形胶质细胞的marker，即不分化为星形胶质细胞；LM：Tom+细胞中：NG2+数量和比率减少，而APC+相反MorethantwothirdsofthedetectedBrdU+/EdU+cellsexpressedNG2(Fig.3B,C),indicatingthatadulthypothalamicNG2-gliacanundergomultipleself-renewingdivisions,inlinewithfindingsforthecerebralcortex[11]；oneoftheprimaryfunctionsofadulthypothalamicNG2-gliaistoprovideacontinuoussupplyofnewoligodendrocytes；FigureS1FidelityoftheNG2creER:tdTomatoreporter.MiceweretreatedwithTXtwicedailyfortwodays.(A)CelltypesweredeterminedbycolocalisationofTomwithantibodiestoNG2toidentifyNG2-glia(arrowheadN),CD13toidentifypericytes(arrowheadP)andAPCtoidentifyoligodendrocytes(arrowheadO).(B)QuantificationofresultsfromA.LabellingwithantibodiestoHuC/D(C)andNeuN(D)confirmedthatTom+neuronswerenotpresentatthistimepoint.Co-labellingwithNG2andSox2antibodiesindicatedthat37.0%+2.8ofhypothalamicNG2-gliarobustlyexpressSox2(Fig.2A,B,F).DespitethefactthatNG2-gliaaccountedforfewerthan10%ofthetotalSox2+cellpopulation,almostall(92.2%+2.8)ofthehypothalamicBrdU+/Sox2+cellsalsoexpressedNG2(Fig.2A,B,F),suggestingthatNG2-gliaaretheonlysubstantiallyproliferativesubpopulationofSox2+cellsintheadulthypothalamicparenchyma.threetypesofNG2-gliapairsaccording