长春工业大学本科毕业论文PAGEI摘要蛹虫草属真菌类物质，其菌丝体具有很高的药用价值和营养价值，尤其是菌丝体中含有的多糖应用十分广泛并有较高的应用价值和开发潜力。实验以蛹虫草菌丝体为原材料，以多糖的提取率为考察指标，比较了热水浸提法，超声波提取法和微波提取法对蛹虫草菌丝体中多糖的提取效果，并利用苯酚－硫酸法测定其多糖含量。实验结果表明，热水浸提法对菌丝体多糖的提取效果最佳，其次是超声波提取法和微波提取法。并采用正交实验法对影响有效成份提取的工艺条件进行了优化，最终确定蛹虫草菌丝体多糖提取的最佳工艺条件为：提取温度80℃，提取时间3h，提取次数为3次，液料比为10:1。关键词：蛹虫草菌丝体；多糖；提纯；正交试验法长春工业大学本科毕业论文长春工业大学本科毕业论文AbstractAsthefungussubstance，theculturedmyceliaofCordycepsmilitarishasahighmedicalandnutritionalvalue,especiallyitspolysaccharidehasbeenwidelyused.Ithasahighapplicationvalueanddevelopmentpotential.TheexperimentregardstheculturedmyceliaofCordycepsmilitarisastheresearchmaterial，theextractionrateofpolysaccharidesasindicators.ToinvestigatethemethodappliedintheextractionofpolysaccharidesinCordycepsmilitarismycelia．Water-extractionmethod，ultrasonicwaveextractionandmicrowaveextractionmethodwereappliedintheextractionofpolysaccharides．Thecontentofpolysaccharidesinmyceliawasdeterminedbyphenol-vitriolmethod．Theexperimentresultshowsthatthecoarsepolysaccharidesextractiverateofwater-extractionmethodwasthebestinCordycepsmilitarismycelia，followedbyultrasonicwaveextractionandmicrowaveextractionmethod.Orthogonaldesignwasbeusedtoassesstheprocessconditionswhichinfluencingtheextractionandseparation,andfinaloptimizedconditionsforextractingexopolysaccharidewasestablished,extracted3hourson80℃，extraction3timesandliquidtosolidration10:1.Keywords:culturedmyceliaofCordycepsmilitaris；polysaccharide；purification；Orthogonaldesignmethod目录TOC\o"1-3"\h\z\uHYPERLINK\l"_Toc294898454"摘要PAGEREF_Toc294898454\hIHYPERLINK\l"_Toc294898455"AbstractPAGEREF_Toc294898455\hIIHYPERLINK\l"_Toc294898456"第一章前言PAGEREF_Toc294898456\h1HYPERLINK\l"_Toc294898457"第二章文献综述PAGEREF_Toc294898457\h2HYPERLINK\l"_Toc294898458"2.1蛹虫草菌丝体PAGEREF_Toc294898458\h2HYPERLINK\l"_Toc294898459"2.1.1概述PAGEREF_Toc294898459\h2HYPERLINK\l"_Toc294898460"2.1.2成分PAGEREF_Toc294898460\h3HYPERLINK\l"_Toc294898461"2.2蛹虫草菌丝体多糖PAGER