长春工业大学本科毕业论文摘要蛹虫草属于真菌类物质，人工蛹虫草子实体具有很高的药用价值和营养价值，尤其是人工蛹虫草子实体中含有的核苷类物质，它的应用十分广泛并有极高的应用价值和开发潜力。本实验以人工蛹虫草子实体为原料，以核苷类物质的提取率为考察指标，比较了超声波提取法、热回流提取法和微波提取法对核苷类物质的提取效果，并对提取溶剂进行了考察。实验结果表明：以水为提取溶剂，超声波提取法效果最佳。正交试验法优化超声波提取的最佳工艺条件为：液料比为40:1、提取温度80℃、超声功率为100W、提取时间40min、提取次数3次。在最优提取条件下，采用HPLC法测定人工蛹虫草子实体腺苷和虫草素的含量分别为0.197%和0.148%。关键词：蛹虫草子实体；腺苷；虫草素；超声波提取；HPLCAbstractAsthefungussubstance，theculturedmyceliaofCordycepsMilitarishasahighmedicalandnutritionalvalue,especiallyitsnucleosidehasbeenwidelyused.Ithasahighapplicationvalueanddevelopmentpotential.TheexperimentregardstheculturedmyceliaofCordycepsMilitarisastheresearchmaterial，theextractionrateofnucleosideasindicators.ToinvestigatethemethodappliedintheextractionofnucleosideinCordycepsmilitarismycelia．Water-extractionmethod，ultrasonicwaveextractionandmicrowaveextractionmethodwereappliedintheextractionofnucleoside．Andselectedthebestextractionsolvent.Theexperimentshowsthatthemethodofmicrowaveextractionisthebest,andthebestextractionsolventiswater.TheexperimentusethemethodofHPLCtodeterminationofcordycepsmilitarisartificiallyfruitbodyandthecontentofChinesecaterpillarfungusadenosinemeal.Orthogonaldesignwasbeusedtoassesstheprocessconditionswhichinfluencingtheextractionandseparation,andfinaloptimizedconditionsforextractingnucleosidewasestablished,thengetthebestconditionis:Liquidmaterialthanis40:1、thetemperatureis80℃、thetimesis3、thetimeis40min.Keywords：Cordyceps；Adenosine；Cordycepin；HPLC；Ultrasonicextraction；目录TOC\o"1-4"\h\uHYPERLINK\l"_Toc327282750"摘要PAGEREF_Toc327282750\h1HYPERLINK\l"_Toc327282751"AbstractPAGEREF_Toc327282751\h2HYPERLINK\l"_Toc327282752"第一章前言PAGEREF_Toc327282752\h5HYPERLINK\l"_Toc327282753"第二章文献综述PAGEREF_Toc327282753\h6HYPERLINK\l"_Toc327282754"2.1蛹虫草子实体PAGEREF_Toc327282754\h6HYPERLINK\l"_Toc327282755"2.1.1蛹虫草子实体的营养成分分析PAGEREF_Toc327282755\h6HYPERLINK\l"_Toc327282756"2.1.2蛹虫草子实体的功能作用PAGEREF_Toc327282756\h6HYPERLINK\l"_Toc327282757"2.1.3蛹虫草子实体的应用PAGEREF_T