胸膜肺炎放线杆菌双组分调控系统QseB/QseC突变株构建功能研究的开题报告摘要近年来，胸膜肺炎放线杆菌（Klebsiellapneumoniae，KP）感染的严重性日益增加，尤其是多重耐药的KP感染更是成为医学领域面临的重要挑战。KPQseB/QseC双组分调控系统在细菌的适应性反应、生长、代谢、致病性等多个重要生理和生化过程中起到关键作用。然而，针对KPQseB/QseC双组分调控系统的突变株研究仍然较少。因此，本研究旨在构建KPQseB/QseC双组分调控系统突变株并对其功能进行研究。本研究将基于质粒的克隆技术构建KPQseB/QseC突变株。利用PCR法将KPQseB/QseC双组分调控系统中的关键基因进行敲除或突变，同时将荧光素酶（GFP）等外源基因靶向至KPQseB/QseC突变株的某些位点，用于追踪并鉴定其功能变化。通过Westernblotting、转录组分析等方法对构建的KPQseB/QseC突变株进行确认和鉴定。本研究预计通过构建KPQseB/QseC突变株探究其在感染中的作用机制和功能，为KP感染的治疗提供新的理论基础和治疗策略。关键词：胸膜肺炎放线杆菌；QseB/QseC双组分调控系统；突变株构建；功能研究AbstractInrecentyears,theseverityofKlebsiellapneumoniae(KP)infectionisincreasing,especiallymulti-drugresistantKPinfectionhasbecomeanimportantchallengeinthemedicalfield.KPQseB/QseCtwo-componentregulatorysystemplaysakeyroleinmanyimportantphysiologicalandbiochemicalprocessessuchasadaptiveresponse,growth,metabolism,pathogenicity,etc.However,studiesonmutantstrainstargetingKPQseB/QseCtwo-componentregulatorysystemarestilllimited.Therefore,thisstudyaimstoconstructKPQseB/QseCmutantstrainsandstudytheirfunctions.Thisstudywilluseplasmid-basedcloningtechnologytoconstructKPQseB/QseCmutantstrains.PCRmethodwillbeusedtoknockoutormutatekeygenesinKPQseB/QseCtwo-componentregulatorysystem,andexogenousgenessuchasgreenfluorescentprotein(GFP)willbetargetedtosomesitesofKPQseB/QseCmutantstrainstotrackandidentifytheirfunctionalchanges.Westernblotting,transcriptomeanalysis,andothermethodswillbeusedtoconfirmandidentifytheconstructedKPQseB/QseCmutantstrains.ThisstudyisexpectedtoexplorethemechanismandfunctionofKPQseB/QseCtwo-componentregulatorysystemininfectionthroughtheconstructionofKPQseB/QseCmutantstrains,andprovideanewtheoreticalbasisandtreatmentstrategyforthetreatmentofKPinfection.Keywords:Klebsiellapneumoniae;QseB/QseCtwo-componentregulatorysystem;mutantstrainconstruction;functionalstudy