采血安乐死麻醉，这么全的实验动物指导方针一定要收藏！导读常见实验动物指导方针：一、常见动物血液采集二、安乐死的指导方针三、常见动物麻醉的指导方针一、常见动物血液采集网站：/services-name/research-animal-resources/research-support/guidelines/blood-collection本网站收集的常见动物有以下：（点击各动物前的+号可以查看详情）1、小鼠血液采集2、大鼠血液采集3、小鼠和大鼠血液采集总结4、兔血液采集5、啮齿动物常见的采血程序介绍点击各动物前的+号，可以查看详细的采血步骤。二、安乐死的指导方针网址：/services-name/research-animal-resources/research-support/guidelines/euthanasia本网站收集的常见动物有以下：（点击对应的物种，可以跳转到查看具体的安乐死方式）1、老鼠、老鼠、沙鼠、仓鼠或其他小啮齿动物安乐死方式①Carbondioxide(CO2)Inhalationtoeffect.Flowratemustdisplacenomorethan30%ofthechambervolume/minute.Prolongedexposuremayberequiredforneonatesandfetuses.②Sodiumpentobarbital（戊巴比妥钠）≥100mg/kgintraperitoneal(IP)orintravenous(IV).Thisisacontrolledsubstance(DEAScheduleII).DosingisthesameforthecommercialproductFatal-Plus®.③Euthanasiasolution（安乐死溶液）≥86mg/kgIPorIV.Multiplepharmaceuticalgradeproductsavailable.Containssodiumpentobarbital390mg/ml+sodiumphenytoin50mg/ml(dosingbasedonbarbiturateconcentration).Thisisacontrolledsubstance(DEAScheduleIII).④Cervicaldislocation（颈椎脱位）Useinconscioussmallrodentsrequiresdemonstratedtechnicalskill(ormustbeperformedonlyonunconscious/anesthetizedsmallrodents).Useinrats>200gtobeperformedunderanesthesiaonly.⑤Decapitation（断头）Useinconscioussmallrodentsrequiresdemonstratedtechnicalskill(ormustbeperformedonlyonunconscious/anesthetizedsmallrodents).Useinrats>200gtobeperformedunderanesthesiaonly.⑥Isofluraneoverdose（异氟醚过量）Inhalationtoeffect.Mayrequireprolongedexposuretoensuredeath.⑦Injectableanestheticoverdose（注射麻醉过量）Administrationvaries(IPorIV).ConsultaResearchAnimalResources(RAR)veterinarianregardingspecificagents.⑧Anesthesiafollowedbypotassiumchloride（麻醉后氯化钾）75-150mg/kgKClIV.Administerrapidlytoeffect.⑨Anesthesiafollowedbyphysicalmethod（麻醉后物理方法）Cervicaldislocation,decapitation,exsanguination,bilateralpneumothorax.2、兔安乐死方式①Sodiumpentobarbital（戊巴比妥钠）≥100mg/kgIPorIV.Thisisacontrolledsubstance(DEAScheduleII).Dosingisthesameforthecommercialprod