香鳞毛蕨治疗银屑病的药效物质基础研究的开题报告Title:StudyontheTherapeuticEfficacyandPharmacologicalBasisofXianglinmaojueintheTreatmentofPsoriasisIntroduction:Psoriasisisachronicinflammatoryskindiseasethataffectsmillionsofindividualsworldwide.Despitetheavailabilityofvarioustreatmentoptions,suchastopicalagents,systemicmedications,andbiologics,thetreatmentofpsoriasisremainschallengingduetoitscomplexpathophysiology,geneticpredisposition,andvariableclinicalpresentation.Therefore,thereisaneedfornewsafeandeffectivetreatmentsforpsoriasis.TraditionalChinesemedicine(TCM)haslongbeenusedtotreatvariousskindiseases,includingpsoriasis.Xianglinmaojue(XL)isatypeofTCMderivedfromtherhizomeofDryopterisfragrans,whichhasbeenshowntohavepotentialtherapeuticeffectsonpsoriasis.Hypothesis:XLexertsitstherapeuticeffectsonpsoriasisbymodulatingimmuneresponsesandregulatingkeratinocyteproliferationanddifferentiationthroughitsactiveconstituents.Objectives:1.ToinvestigatethetherapeuticefficacyofXLonpsoriasisinananimalmodel.2.ToidentifytheactiveconstituentsofXLusinghigh-performanceliquidchromatography(HPLC)andgaschromatography-massspectrometry(GC-MS).3.Toevaluatetheimmunomodulatoryandanti-proliferativeeffectsofXLanditsactiveconstituentsonpsoriasis.Methods:1.Animalmodel:Psoriasis-likelesionswillbeinducedinmicebytopicalapplicationofimiquimod(IMQ)creamtotheshavedbackskin.Themicewillberandomlyassignedtothreegroups:(1)XLtreatmentgroup,(2)IMQ-alonegroup,and(3)vehiclecontrolgroup.TheXLtreatmentgroupwillreceivedailyoraladministrationofXLextractatadoseof500mg/kgbodyweight.TheIMQ-alonegroupwillreceivetopicalapplicationofIMQcreamonly.Thevehiclecontrolgroupwillreceivetopicalapplicationofvehiclecreamonly.Thetreatmentdurationwillbe7days,andtheseverityofskinlesionswillbeassessedusingthePsoriasisAreaandSeverityIndex(PASI)andhistopathologicalanalysis.2.Identificationofactiveconstituents:XLextractwillbeanalyzedusingHPLCandGC-MStoidentifythechemicalconstituents.Theidentifiedcompoundswillbefurthertestedfortheirimmunomodulatoryandan