中国组织工程研究与临床康复第15卷第5期2011–01–29出版JournalofClinicalRehabilitativeTissueEngineeringResearchJanuary29,2011Vol.15,No.5灌注速度对移植肝脏缺血再灌注的损伤**肖宏，尹思能，陈安平，田刚，陈先林，龙飞伍Effectsofperfusionspeedongraftischemia/reperfusioninjuryfollowinglivertransplantationXiaoHong,YinSi-neng,ChenAn-ping,TianGang,ChenXian-lin,LongFei-wuAbstractDepartmentofBACKGROUND:PreviousstudiesdemonstratedthatperfusionpressureinfluencestheenergymetabolismofgraftandaffectsHepatobiliaryitsenergy,suitableperfusionpressurecansignificantlyimprovethequalityofdonor.Surgery,theSecondOBJECTIVE:People’sHospitalofToinvestigatetheeffectsofvariousperfusion-speedsonreperfusioninjuryofgraftafterratorthotopicliverChengdu,Chengdutransplantation.610017,SichuanMETHODS:SDtoSDratorthotopiclivertransplantationmodelswereestablishedbyimprovedKamadatwo-cufftechnique.Province,ChinaPerfusion-speedduringharvestinggraftswas50,100,150and200mL/h,respectively.ThemorphologicalchangeofgraftsXiaoHong,Associatewereobservedbylightmicroscope,alaninetransarninase(ALT)andtumornecrosisfactor-α(TNF-α)inperipheralserumandchiefphysician,endothelialnitricoxidesynthase(eNOS)proteinandmRNAintragraftweredetected.DepartmentofRESULTSANDCONCLUSION:Thepathohistologicaldamageofgraftsin200mL/hgroupwasobviouslycomparedwithotherHepatobiliarygroups.Comparedwith50and100mL/hgroups,ALTin150and200mL/hgroupswassignificantlydecreased(P<0.05,P<Surgery,theSecondPPeople’sHospitalof0.01),buttheexpressionofeNOSproteinandmRNAwasobviouslylower(<0.01).ThehepaticfunctiondamagedseriouslyChengdu,Chengduwithincreasingofperfusionspeedafter100mL/h.Thefindingsdemonstratedthatsuitableperfusionspeedcanreducehepatic610017,Sichuanfunctioninjuryandimproveprognosis.100mL/hwascompatiblespeedforharvestinggraftofratlivertransplantation.Province,ChinaCorrespondenceto:XiaoH,YinSN,ChenAP,TianG,ChenXL,LongFW.Effectsofperfusionspeedongraftischemia/reperfusioninjuryfollowingLongFei-wu,Master,livertransplantation.ZhongguoZuzhiGongchengYanjiuyuLinchuangKangfu.2011;15(5):788-