·488·解放军医学杂志2003年10月第28卷第10期MedJChinPLAVol28No10October2003感觉神经肽SP对创面表皮干细胞迁移影响的实验研究①400042重庆第三军医大学大坪医院黄晖赖西南②王正国付小兵③王丽丽摘要目的探讨感觉神经肽P物质(SP)对创面表皮干细胞迁移的影响。方法新生3～4天Wistar大鼠90只,随机分为正常组、SP组、辣椒素组,采用BrdU标记干细胞结合检测表皮干细胞特异性标记蛋白K19和β1整合素表达,观察皮肤全层缺损后21天伤口局部应用SP对伤口表皮干细胞迁移及伤口愈合的影响,并与预先注射辣椒素毁损感觉神经元(辣椒素组)和单纯皮肤损伤(正常组)比较。结果致伤后18天SP组伤口闭合,较正常组提前3天,而辣椒素组仅闭合创面面积的25154%。伤后SP组创缘及肉芽组织出现较多表皮干细胞;而抑制感觉神经肽SP的辣椒素组仅在创缘观察到少量表皮干细胞;正常组在肉芽组织中尚未发现表皮干细胞,在创缘的表皮干细胞数介于SP组和辣椒素组之间。结论感觉神经肽SP能明显加快创面愈合速度,并具有诱导表皮干细胞向创缘和肉芽组织中迁移的作用。关键词表皮干细胞;感觉神经肽;P物质;溴脱氧尿苷;伤口愈合;迁移中国图书资料分类号R329.2+1EFFECTOFSENSORYNEUROPEPTIDEONMIGRATIONOFEPIDERMALSTEMCELLSINWOUNDREPAIRHuangHui,LaiXinan,WangZhengguoetal.DapingHospital,ThirdMilitaryMedicalUniversity,Chongqing400042,ChinaAbstractObjectiveTostudytheeffectofneuropeptideonmigrationofepidermalstemcellsinwoundrepair.Methods90newbornrats(3～4dold)wererandomlydividedintothreegroups:SPgroup,capsaicingroup,andnormalgroup.BrdUlabeling,combinedwithspecificproteinmarkersofepidermalstemcells,K19andβ1integrin,wereusedtoidentifyepidermalstemcells.Themigrationofepidermalstemcellswasobservedinfullthicknessskinwoundonbackon21daysafterinjury.SubstancePwasappliedtotheskinwoundafterinjuryinSPgroup.Thestatusofmigra2tionofepidermalstemcellsinSPgroupwascomparedwiththatincapsaicingroup,inwhichcapsaicinwasinjectedtodestroysensoryneuronbeforeskininjury,andwithnormalgroup,inwhichtheskinwoundwasnottreatedwithanymedication.ResultsWoundsofratsinSPgroupwerehealed18daysafterinjury.Itwasshortenedbythreedayscomparedwithnormalgroup.Only25154%ofwoundareawashealedincapsaicingrouponday18.ThereweremanyepidermalstemcellsintheedgeofthewoundandgranulationtissueinGroupSP.Onlyasmallnumberofepidermalstemcellswereseenincapsaicingroup,whereSPreleasewasblockedbychemicaldestructionofsensoryneurons,inthewoundedge,butnotingranulationtis2sue.Theamountofepidermalstemcellsasseeninthewoundedge,butnotingranulationtissue,innormalgroupwaslessthanintheSPgroupbutmorethanincapsaicingroup.ConclusionsSPobviouslypromoteswo