浅论妊娠高血压综合征患者血清中抗氧化物与胎盘Bax、Bcl【摘要】目的探讨妊娠高血压综合征(PIH)患者外周血清中抗氧化物和胎盘组织细胞Bcl-2、Bax蛋白表达水平的关系。方法检测68例PIH病人(PIH组)和25例正常孕妇(NLP组)血清中GSH-px、MDA、SOD活性,并用免疫组织化学SP法检测胎盘组织Bax、Bcl-2蛋白并进行比较。结果PIH中、重度组血清GSH-px/MDA、SOD/MDA、Bcl-2/Bax比率明显低于NLP组(P＜);PIH中、重度组胎盘组织细胞凋亡指数、Bax高于NLP组(P＜);各组P53蛋白均未见阳性表达。血清GSH-px/MDA、SOD/MDA与凋亡指数、Bcl-2/Bax呈正相关(P＜)。结论PIH的发生与机体氧化和抗氧化平衡失调及与其引起的胎盘组织细胞凋亡增加有关。【关键词】妊娠高血压综合征氧化和抗氧化平衡Bax蛋白P53蛋白RelationshipBetweenSerumAntioxidantsandtheExpressionofBcl2、BaxApoptosisProteinofPlacentalTissueswithPregnancyinducedHypertensionSyndromeofBasicMedicine,NingxiaMedicalCollege,Yinchuan750004;HealthCareHospitalforWomenandChildren,Yinchuan750001Abstract:ObjectiveToexploretherelationshipbetweensuperoxidedismutase(SOD),glutathioneperoxidase(GSHpx)andmalondialdehyde(MDA)inperipheralvenousbloodofpatientsandtheexpressionofBcl2、Baxapoptosisproteinofplacentaltissueswithpregnancyinducedhypertensionsyndrome(PIH).MethodsTheactivityofSOD,GSHpxandcontentofMDAintheserumofperipheralbloodofnonpregnantwomen,normalpregnantwomenandPIHwomenweremeasuredandtheexpressionofBcl2,Baxwereassessedbyimmunolistochemicaldetection.ResultsTheratioofGSHpx/MDA、SOD/MDA、Bcl2/Baxinnormalpregnantwomenwassignificantlylowerthanthoseinnonpregnantwomen(P＜),apoptosisindex(AI)andtheexpressionofBaxinnormalpregnantwomenweresignificantlyhigherthanthoseinnonpregnantwomen(P＜)；thereweresignificantlypositivecorrelationintheratiobetweenGSHpx/MDA、SOD/MDAandBcl2/Bax、apoptosisindex().ConclusionThemechanismofPIHisassociatedwiththedisequilibriumofoxidation,antioxidationandapoptosisoverexpressionofplacentaltissuesinducedbyit.Keywords:pregnancyinducedhypertension；disequilibriumofoxidationandantioxidation；Bax；P53妊娠高血压综合征(pregnancy-inducedhypertension，PIH)是影响母儿围生期发生率和死亡率的重要因素之一,其病因及发病机制至今尚未完全阐明。大量实验证明PIH患者胎盘组织凋亡增加［1］，细胞凋亡由一系列基因调控,其中促凋基因Bax和抑凋基因Bcl-2被认为是非常重要的成员,Bcl-2/Bax的比率决定了细胞对刺激的敏感性。为此,本实验检测PTH胎盘组织细胞的凋亡、凋亡相关基因Bax、Bcl-2蛋白,以及PIH患者血清中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-px)活性及丙二醛(MDA)含量的变化,旨在探讨SOD、GSH-px、MDA和Bax、Bcl-2的相关性及在PIH发病中的作用，为PIH的防治提供理