不同浓度氯化钴诱导小鼠海马神经元细胞缺氧损伤模型的比较肖婷婷1，王莉1，曹相玫2，倪新莉1*(宁夏医科大学:1.临床医学院，2.基础医学院，银川750004)基金项目:国家自然科学基金地区项目（81660229）;宁夏科技创新领军人才项目（KJT2015017）*通讯作者:倪新莉电话:13909586966;E-mail:1284813176@qq.com;【摘要】目的:研究不同浓度的氯化钴(CoCl2)诱导小鼠海马神经元细胞（HT22）缺氧损伤模型的比较。方法:用不同浓度(0、50、100、200μmol/L)CoCl2建立HT22细胞缺氧模型。通过HE染色观察HT22细胞轴突生长的特征，CCK8法检测HT22细胞体外增殖活性的变化，同时，WesternBlot检测HT22细胞中HIF-1α、tau5的表达情况。结果:HE显示CoCl2可致HT22细胞轴突断裂，CCK8显示CoCl2对HT22细胞增殖抑制作用表现出浓度依赖性。WesternBlot显示CoCl2浓度为200μmol/L时,HIF-1α、Tau5蛋白表达与对照组相比差异有统计学意义。结论：CoCl2可剂量依赖性上调HT22细胞HIF-1α表达，致轴突损伤及tau5降低，建立细胞缺氧模型简单易行。【关键词】HT22细胞;氯化钴;轴突；HIF-1αComparisonofhypoxicinjurymodelsofhippocampalneuronsinducedbydifferentconcentrationsofcobaltchlorideinmiceXiaoTingting1,WangLi1,CaoXiangmei2,NiXinli1*(1.HYPERLINK"C:/Users/dell/AppData/Local/youdao/dict/Application/8.7.0.0/resultui/html/index.html"\l"/javascript:;"SchoolHYPERLINK"C:/Users/dell/AppData/Local/youdao/dict/Application/8.7.0.0/resultui/html/index.html"\l"/javascript:;"ofHYPERLINK"C:/Users/dell/AppData/Local/youdao/dict/Application/8.7.0.0/resultui/html/index.html"\l"/javascript:;"ClinicalHYPERLINK"C:/Users/dell/AppData/Local/youdao/dict/Application/8.7.0.0/resultui/html/index.html"\l"/javascript:;"Medicine,2.SchoolofBasicMedicine,，NingxiaMedicalUniversityYinchuan750004,China)[Abstract]:Objective:Tocomparethehypoxiainjurymodelofmousehippocampalneuroncells(HT22)inducedbydifferentconcentrationsofcobaltchloride(CoCl2).Methods:ThehypoxiamodelofHT22cellswasestablishedwithdifferentconcentrations(0,50,100,200μmol/L)ofCoCl2.ThecharacteristicsofaxongrowthofHT22cellswereobservedbyHEstaining.TheCCK8methodwasusedtodetectthechangesofHT22cellproliferationactivityinvitro.Atthesametime,WesternBlotwasusedtodetecttheexpressionofHIF-1αandtau5inHT22cells.Results:HEshowedthatCoCl2couldcauseaxonruptureinHT22cells,andCCK8showedthatCoCl2hadaconcentration-dependentinhibitioneffectonHT22cellproliferation.WesternBlotshowedthatwhentheconcentrationofCoCl2was200μmol/L,theexpressionsofHIF-1αandTau5proteinweresign