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G25脱盐柱使用指南MatrixSephadex™G-25Fine,cross-linkeddextranBedvolume53mlBedheight100mmi.d.26mmseparationofhigh(Mr>5000)fromlowmolecularweightsubstances(Mr<1000).Storage+4to+30°20%ethanol5大家应该也有点累了，稍作休息SephadexG-10iswellsuitedfortheseparationofbiomoleculessuchaspeptides(Mr>700)fromsmallermolecules(Mr<100).SephadexG-50issuitablefortheseparationofmoleculesMr>30000frommoleculesMr<1500suchaslabeledproteinorDNAfromunconjugateddyes.Themediumisoftenusedtoremovesmallnucleotidesfromlongerchainnucleicacids.SephadexG-25isrecommendedforthemajorityofgroupseparationsinvolvingglobularproteins.ThesemediaareexcellentforremovingsaltandothersmallcontaminantsawayfrommoleculesthataregreaterthanMr5000.samplevolumesofupto30%ofthetotalvolumeofthedesaltingcolumncanbeprocessed.notexceedapproximately70mg/ml。Column:HiTrapDesalting,1×5ml,3×5ml,5×5mlSample:2mg/mlBSAin50mMsodiumphosphate,0.5Msodiumchloride,pH7.0Samplevolume:28%×Vt(1.4,4.3and7.1mlrespectively)Buffer:50mMsodiumphosphate,0.15Msodiumchloride,pH7.0Flowrate:5ml/min
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