中国卫生检验杂志2010年8月第20卷第8期ChineseJournalofHealthLaboratoryTechnology,Aug2010;Vol20No81871=化学测定方法>电位滴定与人工滴定测定消毒灭菌剂中戊二醛含量的比较朱军生,梁建生,刘小丽,徐云斌,邓兵,徐桂兰(武汉市疾病预防控制中心,武汉430015)[摘要]目的:电位滴定测定消毒灭菌剂中戊二醛的含量,并与人工滴定的酸碱滴定法比较。方法:以0.25mol/L硫酸溶液为标准滴定液,分别采用电位滴定和酸碱滴定法测定消毒灭菌剂中戊二醛的含量,并将测定结果计算变异系数、建立直线回归方程。结果:对2.0%~2.2%的戊二醛含量进行测定时,两种方法在相关试剂静置反应时间为60~90min时反应平稳、戊二醛含量稳定。电位滴定变异系数(0.08%)明显低于人工滴定(0.60%),其中电位滴定法在戊二醛含量为0.5%~2.0%的范围内,与硫酸滴定液呈完全的线性相关(r=1.000)。结论:采用电位滴定法测定消毒灭菌剂中戊二醛的含量,具有终点容易判断、误差小、精密度高等优点,值得推广应用。[关键词]电位滴定;人工滴定法;戊二醛;含量测定[中图分类号]O655.22[文献标识码]A[文章编号]1004-8685(2010)08-1871-02Comparisondeterminationofglutaraldehydeconcentrationbetweenpotentiometrictitra-tionandmanualtitrationZHUJun-sheng,LIANGJian-sheng,LIUXiao-li,XUYun-bin,DENGBing,XUGui-lan(WuhanCentreforDiseaseControlandPrevention,Wuhan430015,China)[Abstract]Objective:Itwillusepotentiometrictitrationtodeterminetheglutaraldehydeconcentrationindisinfectionandster-ilizationagent,andcomparewithmanualtitrationoftheacid-basetitration.Methods:With0.25mol/Lsulfuricacidsolutionasthestandardtitrationsolution,itwillrespectivelyusepotentiometrictitrationandacid-basetitrationtodetermineglutaraldehydeconcentrationindisinfectionandsterilizationagent,andalsousetheoutcometocalculatethecoefficientofvariationandestablishthelinearregressionequation.Results:Whenglutaraldehydeconcentrationisbetween2.0%and2.2%,thereactionofthetwomethodsisstablein60~90min.thecoefficientofvariationofpotentiometrictitration(0.08%)issignificantlylowerthanmanualtitration(0.60%),whenglutaraldehydeconcentrationdeterminedbypotentiometrictitrationisbetween2.0%and2.2%itishighlyrelevantwithsulfuricacidtitrationsolution(r=1).Conclusion:Ithasseveraladvantages,suchaseasytojudgetheend-point,littleerror,highprecisionandsoon,whenusingpotentiometrictitrationtodetermineglutaraldehydeconcentrationindisin-fectionandsterilizationagent.Itisworthtospread.[Keywords]Potentiometrictitration;Manualtitration;Glutaraldehyde;Determination戊二醛作为一种冷消毒灭菌剂的出现,是化学消毒灭菌果与人工滴定的酸碱滴定法结