DEK蛋白的表达和纯化及其功能的初步研究的开题报告摘要：DEK蛋白是一种高度保守的核内DNA结合蛋白，参与多种细胞过程如转录和DNA损伤修复。近年来，DEK蛋白在肿瘤发生、进展和预后方面的研究越来越受到重视。本研究旨在构建DEK蛋白表达和纯化系统，并初步探究其功能。首先，利用PCR扩增人DEK基因并克隆到表达载体pET28a(+)，然后通过大肠杆菌BL21(DE3)细胞表达及纯化获得重组DEK蛋白。接着，利用GSTpulldown和电泳移位实验初步探究DEK蛋白在DNA结合及与其他蛋白相互作用中的作用。最后，利用小鼠淋巴瘤细胞株EL4建立DEK蛋白稳定表达细胞系，从而进一步研究其在DNA损伤和肿瘤生物学中的功能。关键词：DEK蛋白；表达；纯化；功能Abstract:DEKproteinisahighlyconservednuclearDNAbindingproteinthatisinvolvedinvariouscellularprocessessuchastranscriptionandDNAdamagerepair.Inrecentyears,researchontheroleofDEKproteinintumoroccurrence,progression,andprognosishasbeenincreasinglyvalued.TheaimofthisstudyistoconstructaDEKproteinexpressionandpurificationsystemandtoexploreitsfunctionpreliminarily.Firstly,thehumanDEKgenewasamplifiedbyPCRandclonedintotheexpressionvectorpET28a(+),andtherecombinantDEKproteinwasobtainedbyexpressionandpurificationinEscherichiacoliBL21(DE3)cells.Then,theroleofDEKproteininDNAbindingandinteractionwithotherproteinswaspreliminarilyexploredusingGSTpull-downandelectrophoreticmobilityshiftassay.Finally,astablecelllineexpressingDEKproteinwasestablishedinmouselymphomacelllineEL4tofurtherinvestigateitsfunctioninDNAdamageandtumorbiology.Keywords:DEKprotein;expression;purification;function