安徽农业科学,JournalofAnhuiAgri.Sci.2008,36(14):5858,5863责任编辑王淼责任校对马君叶不同接种方法对发酵料栽培平菇的影响12翟兴礼，杨霞（1.商丘师范学院生物系，河南商丘476000；2.河南职业技术学院，河南郑州450046）摘要[目的]筛选发酵料栽培平菇适宜的接种方法。[方法]用塑料袋栽培平菇，采用两端开放式接种、菌袋三道接种和菌料拌匀接种3种接种方法，在相同培养条件下，观察其菌丝生长情况和污染率。[结果]两端开放式接种栽培平菇,菌丝长势和子实体的形成都表现出较明显的优势,且污染率极低（7%）。菌料拌匀接种栽培平菇,菌丝生长情况明显比两端开放式接种差，而且污染率较高（21%）；菌袋三道接种与两端开放式接种无明显差别。[结论]用塑料袋栽培平菇，两端开放式接种效果较好，菌丝生长快、长势好、周期短、污染率低。关键词平菇;接种；发酵料；栽培；污染中图分类号S646.1+4文献标识码A文章编号0517-6611（2008）14-05858-01ComparisonofThreeInoculationMethodsinCultivatingPleurotusostreatusbyFermentedMaterialZHAIXing!lietal(BiologyDepartmentofShangqiuNormalUniversity,Shangqiu,Henan476000)Abstract[Objective]ThestudyaimedtoscreenedouttheoptimuminoculatingmethodforPleurotusostreatuscultivatedbyfermentedmaterial.[Method]Pleurotusostreatuscultivatedinplasticbagswereinoculatedbythreemethodsofthetwoendopeninginoculation,threepathinoculationonfungusbag(3sharesofstrainswereinoculatedonthemiddleandtwoendsofbag)anduniformlystirringinoculationwithfungusmaterialtoobservethehyphagrowthandpollutionrateundersamecultivatingconditions.[Result]WhenPleurotusostreatuswasculturedbythetwoendopeninginoculation,thegrowthofhyphaandformingoffruitingbodywerethebestwiththelowestpollutionrate(7%).Thegrowthofhyphaculturedbyuniformlystirringinoculationwithfungusmaterialwasmorepoorthanthatbythetwoendopeninginoculationwiththehigherpollutionrate(21%).Theeffectofthreepathinoculationonfungusbaghadnoobviousdifferencefromthatofthetwoendopeninginoculation.[Conclusion]IncultivatingPleurotusostreatusbyfermentedmaterialinplasticbags,themethodoftwoendsopeninghadgoodeffectthatthehyphagrownfastandbetterwithshortgrowthperiodandlowerpollutionrate.KeywordsPleurotusostreatus;Inoculation;Fermentedmaterial;Cultivation;Pollution平菇（Pleurotusostreatus）栽培原料来源广、生产周期适度。短、经济效益高、市场需求大，因此，近几年来，其生产得到1.4试验方法将发酵好的培养料按3种不同处理进行蓬勃发展，栽培面积不断扩大，产量不断提高，已形成继种袋栽，袋子规格是25cm×45cm×0.02cm。将供试的栽培种植业、养殖业和农副产品加工业之后的又一新兴产业，对农掰成黄豆大小的碎块，接种备用，接种菌种量15%。村经济起着重要的作用[1]。由于农村栽培户栽培条件简陋，两端开放式接种：按每袋装料湿重为3kg，装10