Diabetologia12,129-132(1976)DiabetologiabySpringer-Verlag1976HighConcentrationotSomatostatinImmunoreactivityinChickenPancreasG.C.Weir,P.C.Goltsos,E.P.SteinbergandY.C.PatelDiabetesUnitofMassachusettsGeneralHospital,andEndocrineDivision,Tufts-NewEnglandMedicalCenterHospital,Boston,MassachusettsUSASummary.Summary.Extractsofdiscretelobesofchickenpan-extractswereidenticaltothoseofsyntheticcycliccreaswereassayedforsomatostatin(SRIF),insulin,somatostatin(unpublishedobservationsofPatel,andglucagonimmunoreactivity.ThedistributionofReichlinandWeir).Somatostatinimmunoreactivityhormoneconcentrationwascorrelatedappropriatelyhasalsobeenfoundingastrointestinaltissue[12andwiththeknowndistributionofA,B,andD-cells.unpublishedobservationsofPatel,ReichlinandConcentrationsofallthreehormoneswerehighestinWeir].Thecelloforiginofsomatostatininboththethespleniclobe.TheglucagoncontentoftheventralpancreasandgutappearstobetheD-cell[13].Be-anddorsallobeswaslow.EvidenceispresentedthatcauseofthelargenumberofD-cellsintheaviantheSRIF-likematerialinthepancreaticextractspancreasitwasdecidedtoevaluatethesomatostatinisverysimilartosyntheticcyclicSRIF;parallelim-contentofthechickenpancreas.munoassaydisplacementcurvesandsimilarchromatographicelutionprofileswereobtained.TheSRIFconcentrationinchickenpancreasis21timesMaterialsandMethodshigherthanthatfoundintherat.ChickenpancreasmayprovideausefulmodelforstudiesofsomatostatinFive-weekoldWhiteLeghornchickensweresac-physiology.rificedandindividualpancreaticlobes(ventral,dor-sal,third,andsplenic)weredissectedout,weighed,Keywords:Chickenpancreas,somatostatin,insulin,andplacedinicedHankssolution.Assoonaspossibleglucagon.theywereindividuallyhomogenizedwithaTenBroeckpyrexhomogenizerin5mlof2Naceticacid.Tubescontainingthesehomogenateswerethensus-pendedinboilingwaterforfiveminutestoinactivateproteolyticpancreaticenzymesandtheextractsfro-Somatostatin(SRIF)isacyclicfourteenaminoacidzenpendingassay.Beforedilutionforassayaliquotspeptideorig