常用缓冲溶液的配制方法1．甘氨酸–盐酸缓冲液（0.05mol/L）X毫升0.2mol/L甘氨酸+Y毫升0.2mol/LHCI，再加水稀释至200毫升甘氨酸分子量=75.07，0.2mol/L甘氨酸溶液含15.01克/升。pHXYpHXY2.02.42.62.85050505044.032.424.216.83.03.23.43.65050505011.48.26.45.02．邻苯二甲酸–盐酸缓冲液（0.05mol/L）X毫升0.2mol/L邻苯二甲酸氢钾+0.2mol/LHCl，再加水稀释到20毫升邻苯二甲酸氢钾分子量=204.23，0.2mol/L邻苯二甲酸氢溶液含40.85克/升pH(20℃)XYpH(20℃)XY2.22.42.62.83.0555554.0703.9603.2952.6422.0223.23.43.63.855551.4700.9900.5970.2633．乙酸–乙酸钠缓冲液（0.2mol/L）Na2Ac·3H2O分子量=136.09，0.2mol/L溶液为27.22克/升。pH(18℃)0.2mol/LNaAc(毫升)0.3mol/LHAc(毫升)pH(18℃)0.2mol/LNaAc(毫升)0.3mol/LHAc(毫升)2.63.84.04.24.44.60.751.201.802.653.704.909.258.808.207.356.305.104.85.05.25.45.65.85.907.007.908.609.109.404.103.002.101.400.900.604.磷酸盐缓冲液磷酸氢二钠–磷酸二氢钠缓冲液（0.2）Na2HPO4·2H2O分子量=178.05，0.2mol/L溶液为85.61克/升。Na2HPO4·2H2O分子量=358.22，0.2mol/L溶液为71.64克/升。Na2HPO4·2H2O分子量=156.03，0.2mol/L溶液为31.21克/升。pH0.2mol/LNa2HPO4(毫升)0.3mol/LNaH2PO4(毫升)pH0.2mol/LNa2HPO4(毫升)0.3mol/LNaH2PO4(毫升)5.85.96.06.16.26.36.46.56.66.76.86.98.010.012.315.018.522.526.531.537.543.549.555.092.090.087.785.081.577.573.568.562.556.551.045.07.07.17.27.37.47.57.67.77.87.98.061.067.072.077.081.084.087.089.591.593.094.739.033.028.023.019.016.013.010.58.57.05.3磷酸氢二钠–磷酸二氢钾缓冲液（1/15mol/L）Na2HPO4·2H2O分子量=178.05，1/15M溶液为11.876克/升。KH2PO4分子量=136.09，1/15M溶液为9.078克/升。pHmol/L/15Na2HPO4(毫升)mol/L//15KH2PO4(毫升)pHmol/L//15Na2HPO4(毫升)mol/L//15KH2PO4(毫升)4.925.295.916.246.476.646.816.980.100.501.002.003.004.005.006.009.909.509.008.007.006.005.004.007.177.387.738.048.348.678.187.008.009.009.509.759.9010.003.002.001.000.500.250.1005．磷酸二氢钾–氢氧化钠缓冲液（0.05M）X毫升0.2MK2PO4+Y毫升0.2NNaOH加水稀释至29毫升pH(20℃)X(毫升)Y（毫升）pH(20℃)X(毫升)Y（毫升）5.86.06.26.46.66.85555550.3720.5700.8601.2601.7802.3657.07.27.47.67.88.05555552.9633.5003.950