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TheIdentificationandEstablishmentofthePCRAssayofMycoplasmagallisepticumJinanstrainisolatePoultrymycoplasmadiseaseisoneofthemostimportantinfectiousdiseasesanditcausegreatattentioninrecentyears.Especiallyhasahighmorbidityinthebroilerbreedingwhichconditionsiscrudeandsimple.Clinicaldiagnosisandtreatmentaredifficultbecausemycoplasmaandotherbacteriaalwaysmixedinfection,anditoftencausedgreateconomiclosses.AtpresentthecommonmycoplasmaischickenMycoplasmaGallisepticum(MG)andMycoplasmasynoviae(MS).MGmainlycauseChronicRespiratorydiseases(CRD),andaseriousofpneumatocystinflammation.MSmainlycausejointdiseaseofchickensandsubclinicalrespiratorysymptoms.Pneumatocystinflammationandjointdiseaseleadtotheketonebodyabandonrateofbroilersignificantlyincreased.Mycoplasmameleagridis(MM)mainlycauseturkeydiseasesandmakerespiratorysymptomsandbonedeformityofyouthturkey.Duetomycoplasmawaspassedbyeggs,soanykindofmycoplasmainfectionbreedinghens,thehatchingrateoftheeggandsurvivalrateofoffspringchickswillbesignificantlyreduced.MGdoesn'thascytodermandisagram-negativebacteria.Since1933,NelsonisthefirstmanfoundMG,untillnowwehaveacomprehensiveandsystematicunderstandingaboutit.MGcancausechronicrespiratorydiseaseofchickens,thechickenwillhavecough,runnynose,mouthbreathingheavily.ChickenofinfectionMGthatmayhasimmunitydecreasly,easyconcurrentorsecondaryEscherichiacolidisease,Newcastledisease,infectiousbronchitisandinfectiouslaryngotracheitisetc..Accordingtostatistics,comparedwithhealtychicks,thechicksweakcheeperrateofMGinfectionincreasesabout10%,layingratefellby10~20%,broilerweightlossof10to30%.ThecommoditychickenMGinfectionpositiverateis50~80%inourcountry.MGinfectionhasbecomeaseriousquestiononintensifictionchickens.Scholarsbothathomeandabroadhavemanyresearchofthedisease.PoultrymycoplasmaELISAcommoditydiagnostickithasbeensuccessfullydevelopededinmanycountries.HowervemostofthedetectionforserumantibodyandeveryclinicalsamplesneedtotesttheMG,MSatthesametime.Itisexpensiveandnotsuitablefordetectinglarge-scalepur