Chapter1Introduction§1.1Downstreamprocessinginbiotechnology§1.2Demandsonthedownstreamprocessing§1.3Separationmechanismandunitoperation§1.4EstimationforseparationefficiencyChapter2Solid-liquidseparationandcelldisruption§2.1Cellseparation§2.1.1Settling§2.1.2Centrifugation§2.1.3Filtration§2.2Celldisruption§2.2.1Cellstructures§2.2.2Theprinciplesofcelldisruption§2.2.3ThemeansofcelldisruptionAcombinationofenzymatic/chemicallysiswithmechanicaldisintegrationhasbeensuggestedinenhancingtheefficienciesoftherespectivemethods,withsavingsintimeandenergyandthefacilitationofsubsequentprocessing.Chapter3Precipitation§3.1Salting-outprecipitation§3.1.1Theory§3.1.2TheinfluencingfactorstemperatureandpH（to）underahigherionicstrengththesolubilityofproteinswilldecreaseaccompaniedwiththeincreaseoftemperature.§3.1.3Unitoperationofsalting-outplotafiguretodescribethecorrelationbetweentheconcentrationoftotalproteinandthatofgivenproteinandsaturationof(NH4)2SO4inthemotherliquor，bythatdecidetheadditiveamountbasedontherequestforrecoveryofproducts.§3.1.4Application§3.2IsoelectricprecipitationAsaruleitisappliedtoprecipitationofhydrophobicproteinse.g.casein,andnotsuitableforhydrophilicproteinse.g.glutin.Soapplyingfieldsisnotwiderthansalting-outprecipitaion.Characteristic：advantage：itisfacilitatedtosubsequentoperation；disadvantage：sometimesextremesofpHdenaturetheproducts.§3.3Organicsolventprecipitation§3.4Anothermethods§3.4.1Thermalprecipitation§3.4.2SpecialagentsChapter7Affinitypurification§7.1Basicprinciple§7.1.1Molecularrecognitionprocesses§7.1.2Receptor-ligandinteractions§7.2Affinitychromatography§7.2.1Principleandoperation§7.2.2ligand§7.2.3Applicationandadvantage§7.3Affinitymembrane§7.3.1Principleandoperation§7.3.2Advantage§7.4Affinityprecipitation§7.4.1Principle§7.4.2ApplicationChapter4Extraction§4.1Basicconcepts§4.1.1Extraction§4.1.2Back-extraction§4.1.3Physicalextractionandchemicalextraction§4.1.4Distributionlaw§4.2Organicsolventextraction§4.2.1Theinfluencingfactors§4.2.2Operatingmodeandcal