中国农业科学2005,39(12):2597-2602ScientiaAgriculturaSinica动物、环境及饲养员多重耐药大肠杆菌的PFGE分型研究杨玲丽1，陈杖榴1，刘健华1，曾振灵1，马俊英1，吴聪明212（华南农业大学兽医学院/广东省兽药研制与安全评价重点实验室，广州510642；中国农业大学动物医学院，北京100094）摘要：【目的】了解养殖场多重耐药大肠杆菌耐药性的传播机制，探讨人、动物与环境间耐药菌垂直克隆传播的可能性。【方法】采用脉冲场凝胶电泳（PFGE）分型方法对分离自养殖场动物、环境和饲养员的86株耐药谱相近多重耐药大肠杆菌进行DNA指纹图谱分型，比较菌株之间的亲缘关系。【结果】37株鸡场来源大肠杆菌可分为20种PFGE分型；49株猪场来源大肠杆菌可分为24种PFGE分型；同一动物、环境或饲养员采样点分离菌株PFGE指纹图谱相同者较多；同一养殖场不同动物个体、养殖场环境不同采样点、动物和环境以及饲养员和环境都存在PFGE指纹图谱完全相同菌株；鸡场和猪场都发现有属同一PFGE分型的来自动物、饲养员和环境的菌株；86株菌全部对环丙沙星、氨苄西林、链霉素、四环素、多西环素和复方新诺明耐药，相同PFGE分型的菌株其耐药谱型不一定相同。【结论】养殖场动物、环境及饲养员之间存在多重耐药大肠杆菌的克隆传播。关键词：大肠杆菌；多重耐药；PFGE分型；动物；克隆传播MolecularTypingofMultidrug-ResistantE.coliIsolatedfromFarmAnimals,FarmersandEnvironmentbyPulsed-FieldGelElectrophoresisYANGLing-li1,CHENZhang-liu1,LIUJian-hua1,ZengZhen-ling1,MAJun-ying1,WUCong-ming21（CollegeofVeterinaryMedicine,GuangdongKeyLaboratoryforVeterinaryDrugDevelopmentandSafetyEvaluation,South2ChinaAgriculturalUniversity,Guangzhou510642;CollegeofVeterinaryMedicine,ChinaAgriculturalUniversity,Beijing100094)Abstract：【Objective】Toinvestigatethemechanismresponsibleforthedisseminationofmultidrug-resistantE.coliinanimalfarmsandtheclonalrelatenessoftheisolatesfromfarmanimals,farmersandenvironment.【Method】86multidrug-resistantE.coliisolateswithsimilarresistanceprofileswerecharacterizedbypulsed-fieldgelelectrophoresis(PFGE)afterXbaIdigestionanddrugsusceptibilitytesting.【Result】37E.colistrainsfromonepoultryfarmand49E.colistrainsfromoneswinefarmwereclassifiedinto20and24differentsubtypesrespectivelyaccordingtothePFGEprofiles.ThePFGEpatternsofstrainsisolatedfromdifferentsourceswereheterogeneous,thoughstrainsfromthesameanimal,farmerorenvironmentsamplewerequitehomologous.E.coliwithanidenticalPFGEpatternwererecoveredfromstrainscollectedfromdifferentanimalsinthesamefarm,fromanimalsandenvironment,andalsofromfarmersandenvironment.Moreover,E.colistrainswithverysimilarPFGEpatternswerealsoisolatedatbothfarmsfromanimalsandfarmers.Allisolateswereresistantt