毕业设计(论文)课题名称物料预处理对酶法提取灵芝多糖的影响学生姓名学号系、年级专业指导教师职称2015年5月20日PAGE\*MERGEFORMATIII物料预处理对酶法提取灵芝多糖的影响摘要目的:经过对灵芝中灵芝多糖的工艺提取的研究，找出最佳的物料预处理方法处理灵芝，然后进行提取，让灵芝多糖的提取率尽可能的达到最大值。方法:本实验主要采用酶法提取灵芝多糖的方法，以灵芝多糖的提取率为目的，进行各项单因素实验探究，考察物料烘烤温度、物料粒度和紫外线的照射时间等物料预处理的条件对灵芝多糖提取的影响。最后紫外分光光度计测定灵芝多糖的质量，并得出最佳的实验条件。结果:经由实验结果整理分析可以得知，单因素的影响大小为B（C）＞A（A烘烤温度，B物料粒度，C紫外线照射时间），其最佳组合为B2C2A1，粉细度为80目，紫外线照射时间为35min，烘烤温度为75℃，灵芝多糖提取率最高，能够达到1.55%。结论：通过对物料的预处理可以发现，较之未经物料预处理的提取，灵芝多糖的提取率显著提高。关键词:预处理；灵芝多糖；酶法提取；紫外-可见分光光度法ThematerialpretreatmenteffectsonenzymaticextractionofGLP(2011BiologicalEngineering,DepartmentofBiologyandChemistryEngineering,ShaoyangAbstractObjective:TofindoutthebestmaterialpretreatmentextractiontechnologymakestheGLPextractionyieldashighaspossiblebystudyingontheextractionandpurificationofGanodermalucidumpolysaccharides(GLP)fromGanodermalucidum.Methods:Toinvestigatethebakingtemperature,materialsizeandUVprocessingtimeofmaterialpre-treatmentconditionsonextractionofGLPinfluencebasedonsinglefactorexperiment,usingenzymaticextractionmethodandtakingGLP’syieldastheindex.TooptimizetheoptimumconditionsofextractingGLPfromGanodermalucidumpowderbytheorthogonaltest,whichapplyultravioletandvisiblespectrophotometry(UV-vis)todeterminethecontentofGLP.Results:TheresultsoforthogonaltestshowthatthemainfactorswhichinfluenceGLP’syieldinenzymatictextractionwiththematerialpreprocessingareasfollows:BorC>A(Aisthebakingtemperaturet,Bisthematerialsize,CisUVprocessingtime),andthebestparametercombinationisB2C2A1.Thatistosay,Theoptimumconditionsinultrasonicenzymatictextractionwiththematerialpreprocessingarethatthematerialsizeis80mesh,bakingtemperatureis75℃,uvprocessingtimeis35min,andGLP’syieldisupto1.55%.Conclusion:basedonthematerialpretreatmentcanbefoundthatcomparedwiththeextractionofrawmaterialpretreatment,ganodermalucidumpolysaccharidesextractionyieldwassignificantlyimproved.Keywords:Pretreatment;Ganodermalucidumpolysaccharide(GLP);Enzymaticmethod；Uv-visspectrophotometry