EnzymaticPretreatmentonRose-HipOilExtraction:HydrolysisandPressingConditionsJ.Concha,C.Soto,R.Chamy,andM.E.Zúñiga*EscueladeIngenieríaBioquímica,UniversidadCatólicadeValparaíso,Valparaíso,ChileABSTRACT:Inapreviousreport[Zúñiga,M.E.,J.Concha,C.studiedbyseveralauthors(1–8).Theeffectofenzymaticpre-Soto,andR.Chamy,EffectoftheRoseHip(Rosaaff.rubiginosa)treatmentdependsonthestructureoftheoilseedandthecom-OilExtractionCold-PressedProcess,inProceedingsoftheWorldpositionofthecellwall;thus,itvariesaccordingtothekindofConferenceandExhibitiononOilseedProcessingandUtiliza-oilseedandtypeofenzymeused(1).Forrose-hipoilextrac-tion,editedbyR.F.Wilson,AOCSPress,Champaign,2001,pp.tion,therehavebeenreportsontheeffectivenessofenzymatic210–213],theauthorsshowedthatanenzymaticpretreatmentoftreatmentspriortopressinginincreasingtheoilyield(2).rose-hipseeds,priortooilextractionbycoldpressing,improvesMostofthestudiesonenzyme-assistedoilextractiontheoilyield.Inthiswork,westudiedtheeffectsoftemperatureandmoistureduringtheenzymatichydrolysisstageusingtwoprocesseshavebeenperformedusingaqueousextraction.Thispreviouslyselectedmixturesofcommercialenzymes:(i)Olivextechniqueisfrequentlyappliedinruraloilextractionopera-(mainlypectinase)plusCellubrix(mainlycellulase),and(ii)tions,ashasbeendescribedbyseveralauthors(3–8).Inaque-Finizym(mainlyβ-glucanase)plusCellubrix(mainlycellulase)ousprocesses,theenzymaticactionimprovesoilrecoveryby(allfromNovozymesA/S,Madrid,Spain).Inaddition,weevalu-degradingtheseedcellwall,resultingintheruptureoftheatedtheeffectofenzymatichydrolysisontheoilextractionpress-polysaccharide–proteincolloidsystem.ingrateatdifferentoperationalpressures.SampleshydrolyzedInenzyme-assistedoilextractionusingcoldpressing,theenzymaticallybyeitherofthetwocommercialenzymemixturesenzymeactsonlybyhydrolyzingthecellwall,becauseinthisat45°Cand30–40%moistureshowedoilextractionyieldsuptononaqueoussystemthereisnopolysaccharide–proteincolloid.60%,anincreaseofgreaterthan50%,ascomparedwi