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生物技术基础EssentialsofBiotechnologyRequirementsonStudentsHowtolearnCourseArrangement:ContentReferencesPrefaceDefinition&RelatedAreasTransgenicTech转基因技术MetabolicEngineering代谢工程StemCells&TissueEngineering组织工程与干细胞Bio-medicine.生物医药EnvironmentalBiotech.环境生物技术Agric.Biotech.农业生物技术Bio-materials生物材料Bio-fuelEngineering生物能源Bio-leaching生物浸矿SingleCellProtein单细胞蛋白Bioreactors生物反应器Bio-chips生物芯片GeneCloningDNACloningCellEngineeringLee'screatedRuppybyfirstinfectingdogfibroblastcellswithavirusthatinsertedthefluorescentgeneintoacell'snucleus.Transferredtoanotherdog'seggcell,withitsnucleusremoved.Thenimplantedtheclonedembryointoasurrogatemother.Startingwith344embryosimplantedinto20dogs,endedupwithsevenpregnancies.Onefetusdiedabouthalfway,onediedofpneumoniaalive,5werehealthyandspawntheirownfluorescentpuppies.Besidesthelowefficiencyofcloning–just1.7%ofembryoscametoterm–anotherchallengetocreatingtransgenicdogsiscontrollingwhereinthenuclearDNAaforeigngenelands.EnzymeTechnologyNewConceptofEnzymologyIndustrialFermentationThreeComponentsofCoreSectionOverallBiotech.DevelopmentThreeStages:Traditionalbiotech.Brewingtechniqueswhichcouldbetracedbackto5000B.C.,mightbereferredastraditionalbiotech.Developedbiotech.Fermentationandenzymeproductioncouldbeconsideredasdevelopedbiotech..Modernbiotech.includes:cloning,cellengineering,genomeprojects,bio-informatics,etc,.FundamentalofModernBiotech.BasedupontheProfoundUnderstanding:Someofthekeyapproachessince1970s:DNAcloningandsequencingtechnique,DNAsynthesisandamplificationmethods,plantcellengineering,monoclonalantibodytech.,transgenictech.,macromolecularstructureanalysisandprediction,humangenomeproject,bio-informatics(genomics,proteomics).Biotech-productionPrioritiesofBiotechProductioninChinaBio-resources:specialgermlinesoflivestock,agri-plants,appliedmicrobes,herbs,generesources---Bio-techs:longhistoryoffermentationpractice,enzymeproductiontech.genemanipulation---Moreuniv.setbio-techdisciplines.,attractmorestudentstotakethisma
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