西南科技大学本科生毕业论文PAGE\*MERGEFORMAT-38-Southwestuniversityofscienceandtechnology本科毕业设计（论文）醇诱导下番茄果实的防御反应学院名称生命科学与工程学院专业名称生物技术学生姓名学号指导教师醇诱导下番茄果实的防御反应摘要：本文以香叶醇和叶醇诱导的番茄果实为材料，通过测定果实多酚氧化酶活性和防御相关基因的表达，研究了醇诱导下番茄果实的防御反应。结果表明，香叶醇和叶醇诱导能够引起植物的防御性反应。与香叶醇相比，叶醇处理对果实的伤害作用更为明显，引发了更多的多酚氧化酶活性的提高。随着香叶醇或叶醇处理的时间延长，经过醇诱导果实中的CDI、PS及inhII基因的表达量越高;而LOX基因属于早期基因，随着香叶醇或叶醇处理的时间延长，LOX基因的表达量越低。关键词：多酚氧化酶；防御反应；信号途径；醇诱导Defense-relatedresponseinducedbyalcoholapplicationintomatofruitAbstract:Inthispaper,polyphenoloxidase(PPO)activityandtheexpressionofdefense-relatedgenesincludingCDI,PS,LOXandinhIIweredetectedintomatofruitsthatweretreatedwithexogenousgeranioland3-hexenol.Theresultsshowedthatgeranioland3-hexenolcouldtriggerthedefensiveresponseintomatofruits.Comparedwithgeraniol,3-hexenoltreatmenthadobviouswoundingeffectonfruit,triggeringmoreincreasedactivitiesofpolyphenoloxidase.Withtimeextendingofgeraniolor3-hexenoltreatment,theexpressionofCDI,PS,andinhIIgeneinducedbyalcoholwashigher,butsincetheLOXgenebelongstoearlyexpressiongene,theexpressionofLOXinducedbythealcoholwaslowerwithgeraniolor3-hexenoltreatmenttimeprolonging.Keywords:Polyphenoloxidase;Defenseresponse;Signalpathway;alcoholapplication目录TOC\o"1-3"\h\z\uHYPERLINK\l"_Toc359068757"醇诱导下番茄果实的防御反应PAGEREF_Toc359068757\h-2-HYPERLINK\l"_Toc359068758"摘要PAGEREF_Toc359068758\h-2-HYPERLINK\l"_Toc359068759"关键词PAGEREF_Toc359068759\h-2-HYPERLINK\l"_Toc359068760"AlcoholinduceddefenseresponsesintomatofruitPAGEREF_Toc359068760\h-3-HYPERLINK\l"_Toc359068761"AbstractPAGEREF_Toc359068761\h-3-HYPERLINK\l"_Toc359068762"KeywordsPAGEREF_Toc359068762\h-3-HYPERLINK\l"_Toc359068763"第1章绪论PAGEREF_Toc359068763\h-6-HYPERLINK\l"_Toc359068764"1.1植物诱导防御反应相关的主要信号转导途径PAGEREF_Toc359068764\h-6-HYPERLINK\l"_Toc359068765"1.1.1茉莉酸(jasmonieaeid，JA)信号途径PAGEREF_Toc359068765\h-6-HYPERLINK\l"_Toc359068766"1.1.2水杨酸(salicylicacid，SA)信号途径PAGEREF_Toc3590