GhrelinisanOsteoblastMitogenandIncreasesOsteoclasticBoneResorptionInVitroGhrelin(胃动素、胃肠动素)是生长激素促分泌物质受体(growthhormonesecretagoguereceptor,GHS-R)的内源性配体.Ghrelin的结构：Ghrelin是由28个氨基酸组成的小分子多肽，它有两种形式：Ghrelin-28和des-Gln14-ghrelin,除了第14位氨基酸Gln的缺失,des-Gln14-ghrelin生物活性与Ghrelin相同。GhrelinN端第3位丝氨酸残基上的n-辛酰基（n-octanoylgroup）对生物活性具有重要作用。人和大鼠的Ghrelin在进化上高度保守，仅11位和12位上相差两个氨基酸。StructuresofhumanandratghrelinsThestructureofghrelinreceptor,GHSRSignaltransductionpathwaySummary1.IntroductionAnumberofstudiesinvestigatedtheeffectsofghrelininskeletaltissue.AstudyoftheexpressionofGHSR1ainhumantissuesdemonstratedaweakpositivesignalinbonemarrow。Osteoclastresponsetoghrelinhasneverbeeninvestigatedpreviously.Theaimofthecurrentstudywastofurtherinvestigatetheactivityofghrelininbonecells,andinparticularitspossibleroleintheacutestimulationofboneresorptionthatoccursduringfasting.AcylatedghrelinBSA(牛血清白蛋白)GibcobrandFBS(胎牛血清)andmediawereusedTritiatedthymidine（氚化胸腺嘧啶）Tartrate-resistantacidphosphatase(TRAP)stainingkit(抗酒石酸酸性磷酸酶染色试剂盒试)1,25-dihydroxyvitaminD3osteoprotegerin(OPG)（骨保护素）salmoncalcitonin（鲑鱼降钙素）OsteoclastdifferentiationinmousebonemarrowculturesGhrelinincreasedthebone-resorbingactivityofratosteoclastsinvitroResultsOsteoclastactivityinmousecalvarialcultureisnotaffectedbyghrelin.Ghrelinismitogenictoprimaryhumanosteoblasts.Ghrelinweaklyinducedcellproliferationinprimaryratosteoblasts,witha1.1-1.2-foldstimulationofthymidineincorporation(a))andasimilar,modestincreaseincellnumbers(b).Conclusion感谢亲观看此幻灯片，此课件部分内容来源于网络，如有侵权请及时联系我们删除，谢谢配合！