(毕业论文)复方白芍保肝颗粒标志性成分检测方法的研究.docx
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(毕业论文)复方白芍保肝颗粒标志性成分检测方法的研究.docx

(毕业论文)复方白芍保肝颗粒标志性成分检测方法的研究.docx

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长春工业大学本科毕业论文PAGE\*MERGEFORMAT35摘要白芍、葛根、五味子、丹参、甘草是常见的中草药,盛产于中国,对于化学性肝损伤有一定的功效作用。白芍的功效成分为芍药苷,五味子的功效成分为五味子醇甲,丹参的功效成分为丹参酮ⅡA和丹酚酸B,葛根的功效成分为葛根素,甘草的功效成分甘草苷。本论文定性和定量的分析了原药材和成品功效成分的含量及检测方法,研究表明用薄层分析法对原药材和成品进行定性分析,用超声提取对成品进行提取,利用紫外分光光度法对原药材和成品进行定量分析,结果表明原药材符合中华人民共和国药典中规定的含量标准,即原药材合格。经测定成品中每100g含芍药苷800mg,葛根素800mg,五味子醇甲300mg。方法学考察表明紫外分光光度法在测定原药材和成品含量时,方法简便,重现性好,结果准确,稳定性好。关键词:保肝;功效成分;紫外分光光度法;薄层分析法;超声波提取法AbstractWhitepeonyroot,kudzuroot,Schisandra,redsageroot,licoriceisacommonChineseherbs,richinChina,theeffectivenessoftheroleofchemicalliverinjury.Theeffectivenessofthecompositionofthewhitepeonyispaeoniflorin,theeffectivenessofthecompositionoftheSchisandraisschizandrin,theeffectivenessofthecompositionoftheDanshenisTanshinoneⅡAandsalvianolicacidB,theeffectivenessofthecompositionofthePuerariaispuerarin,theeffectivenessofthecompositionofthelicoriceisliquiritin.Inthisthesis,bothqualitativeandquantitativeanalysisofthecontentoftherawherbsandfinishedeffectivenessofthecompositionanddetectionmethods.Thatthequalitativeanalysisofrawherbsandfinishedwiththinlayerchromatography,ultrasonicextractiontoextractthefinishedproduct,quantitativeanalysisonrawherbsandfinishedbyUVspectrophotometry.TheresultsshowthattheoriginalingredientstomeetthecontentstandardssetforthinthePharmacopoeiaofthePeople'sRepublicofChina,thatis,theoriginalmedicinalqualified.Thedeterminationoffinishedproducts:per100gofpaeoniflorinin800mg,per100gofpuerarinin800mg,per100gofschizandrinin300mg.MethodologyinvestigatedshowsUVspectrophotometryinthedeterminationoftheoriginalingredientsandfinishedcontent,themethodissimple,reproducible,accurateandgoodstability.Keywords:liverprotection;functionalcomponents;ultravioletspectrophotometry;thinlayerchromatography;ultrasonicextraction目录TOC\o"1-3"\h\z\uHYPERLINK\l"_Toc327553637"摘要PAGEREF_Toc327553637\hIHYPERLINK\l"_Toc327553638"AbstractPAGEREF_Toc327553638\hIIHYPERLINK\l"_Toc327553639"第一章前言PAGEREF_Toc327553639\h1HYPERLINK\l"_Toc327553640"第二章