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Chapter3ProgressinScienceisOftendrivenbyadvancesinTechnologyThelong-rangegoal1.CellCulture体内、外细胞的差异CompositionofaTypicalMediumWidelyUsedEucaryoticCellLinesInvertedMicroscope多种多样的细胞形态细胞有丝分裂相PrimaryCulture--NeuronCultureFigure8-5MolecularBiologyoftheCell(©GarlandScience2008)CellsCanBeIsolatedfromaTissueSuspensionandSeparatedintoDifferentTypesFigure8-6MolecularBiologyoftheCell(©GarlandScience2008)CellsCanBeFusedTogethertoFormHybridCellsHybridomaCellLinesProvideaPermanentSourceofMonoclonalAntibodies2.FractionationofCellsA.ThetechniqueofdifferentialcentrifugationComparisonofvelocitysedimentationandequilibriumsedimentation.Figure8-73MolecularBiologyoftheCell(©GarlandScience2008)Figure8-23MolecularBiologyoftheCell(©GarlandScience2008)Figure8-21MolecularBiologyoftheCell(©GarlandScience2008)4.ProteinInteractionFigure8-24MolecularBiologyoftheCell(©GarlandScience2008)Figure8-16MolecularBiologyoftheCell(©GarlandScience2008)Figure8-26MolecularBiologyoftheCell(©GarlandScience2008)Figure8-64MolecularBiologyoftheCell(©GarlandScience2008)Figure8-65MolecularBiologyoftheCell(©GarlandScience2008)Hypertrophyandinvadingtumor-likerosettephenotypeofkap3cKOmouseTHANKS!
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