本科毕业论文内蒙古乌拉特中旗传统乳制品中乳酸菌的分离鉴定学院：食品科学与工程专业：食品科学与工程学号：110910787姓名：张永亮指导教师：孟和毕力格职称：教授论文提交日期：二Ο一五年六月摘要传统乳制品是乳酸菌的天然栖息环境，为了认识和研究干旱半荒漠地区传统乳制品中乳酸菌菌群结构，并分离保藏该乳酸菌资源，以采自内蒙古乌拉特中旗的35份传统乳制品样品（19份酸牛奶，7份奶油，2份奶酪，7份鲜牛奶)为研究对象，运用纯培养技术和16SrRNA基因序列分析方法对样品中乳酸菌进行分离鉴定。结果显示：从35份样品中共分离获得属于12个种和亚种的124株乳酸菌，即Lac.lactissubsp.lactis（48株，占分离株38.71%）、L.plantarum（32株，占25.81%）、Leu.mesenteroides（22株，占17.74%）、L.paracasei（6株，占4.84%）、L.helveticus（5株，占4.03%）、L.rhamnosus（3株，占2.42%）和L.brevis、L.diolivorans各2株（分别占1.61%），且Leu.pseudomesenteroide、E.durans、E.sulfurous、L.buchneri各1株（分别占0.81%）。其中以Lac.lactissubsp.lactis和L.plantarum为样品中优势菌群，其次Leu.mesenteroides和L.paracasei的分离数量较多。关键词：传统乳制品；乳酸菌；分离鉴定IsolationandidentificationofLaticacidbacteriafromtraditionaldairyproductsinWulateZhongqiAbstractTraditionaldairyproductswerethenaturalhabitatsoflacticacidbacteria.Inordertorevealandresearchthepopulationstructureoflacticacidbacteria,andpreservelacticacidbacteriafromtraditionaldairyproductsinaridanddesertareas.Lacticacidbacteriawereisolatedandidentifiedusingpureculturemethodand16SrRNAgenesequencingfromsixtyninesamplesoftraditionaldairyproducts,includingyogurt,cream,cheeseandmilk,whichwerecollectedfrompastoralofHYPERLINK"app:ds:Bayan%20Nur%20City"\t"_self"WulateZhongqiareasofInnerMongolia.Theresultsshowedasfollows:124isolatesfrom35samplesofWulateZhongqiofInnerMongoliawereidentifiedinto12speciesorsubspecies,includingLac.lactissubsp.lactis(48strains,accountfor38.71%ofthetotalisolate),L.plantarum(32strains,accountfor25.81%),Leu.mesenteroides(22strains,accountfor17.74%),L.paracasei(6strains,accountfor4.84%),L.helveticus(5strains,accountfor4.03%),L.rhamnosus(3strains,accountfor2.42%)andL.brevis,L.diolivorans(2strains,accountfor1.61%respectively)andLeu.pseudomesenteroide、E.durans、E.sulfurous、L.buchneri(1strains,accountfor0.81%respectively).Lac.lactissubsp.lactisandL.plantarumwerethepredominantspeciesofsamples,thesecondareLeu.mesenteroidesandL.paracasei.Keywords:Traditionaldairyproducts;Lacti