燕窝中唾液酸含量测定方法的研究（完整版）实用资料(可以直接使用，可编辑完整版实用资料，欢迎下载）间的分子机制,有待于进一步研究。参考文献:[1]AlexiouCH,SchmidR,JurgonsR,etal.Targetedtumortherapywith“magneticdrugtargeting”:Therapeuticefficacyofferrofluidboundmi2toxantrone[J].LectureNotesinPhysics,2002,594:2332251.[2]FarokhzadOC,ChengJJ,TeplyBA,etal.Targetednanoparticle2ap2tamerbioconjugatesforcancerchemothe2rapyinvivo[J].ProcNatlA2cadSciUSA,2006,103(16:631526320.[3]ChengTC,ManorekG,SamimiG,etal.Identificationofgeneswhoseexpressionisassociatedwithcisplatinresistanceinhumanovariancar2cinomacells[J].CancerChemotherPharmacol,2006,58(3:3842395.[4]戴永援,陈宝安,王雪梅,等.磁性纳米四氧化三铁及纳米金协同柔红霉素作用于K562/A02细胞的初步研究[J].东南大学学报:医学版,2007,26(3:1572160.[5]ChenBA,SunQ,WangXM,etal.ReversalinmultidrugresistancebymagneticnanoparticleofFe3O4loadedwithadriamycinandtetrandrineinK562/AO2leukemiccells[J].IntJNanomed,2021,3(2:2772286.[6]FerryKV,HamiltonTC,JohnsonSW.Increasednucleotideexcisionrepairincisplatin2resistantorariancancercells:RoleofERCCI2XPF[J].BiochemPharmacol,2000,60(9:130521313.燕窝中唾液酸含量测定方法的研究姜水红,查圣华,谢丽芬,张宏(北京同仁堂健康药业股份,北京100085摘要:目的研究燕窝中唾液酸含量的测定方法,制定燕窝的质量标准。方法燕窝经过磷酸水解,其中的唾液酸(即N2乙酰神经氨酸从唾液酸糖蛋白的结合状态游离出来,用高效液相色谱法进行检测,以唾液酸含量作为评价指标,通过三因素三水平正交试验,优选出最佳的水解条件。结果唾液酸浓度在20~330μg/mL范围内线性良好,平均回收率为98.4%,RSD为1.02%(n=6。结论此法具有稳定性及重复性好、灵敏度高、操作简单等特点,能够准确地检测出燕窝中的唾液酸含量。关键词:燕窝;唾液酸(N2乙酰神经氨酸;高效液相色谱法中图分类号:R927.2文献标识码:A文章编号:100521678(20210520315203Studyonthedeterminationofsialicacidinediblebird′snestJIANGShui2hong,ZHASheng2hua,XIELi2fen,ZHANGHong(BeijingTongrentangHealthPharmaceuticalCo.,Ltd.,Beijing100085,ChinaAbstract:PurposeTostudyonthedeterminationmethodofthecontentofsialicacidinediblebird′snest(EBN,soastoestablishEBNqualitystandard.MethodsAfteracidhydrolysis,thesialicacidinthebird′snest(thatisN2Acetylneuraminicacidisfreefromthecombinationofthestateofglycoproteinsialicacid.Highperformanceliquidchromatography.Isusedtodetectthesialicacidasevaluationindex.Theoptimizedconditionsarefoundonthebasisoforthogonalexperimentsofthreefactorsandthreelevels.ResultsThelinea